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化学学报
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化学学报 ›› 2005, Vol. 63 ›› Issue (8): 783-786. 上一篇    

研究简报

双核钌(II)多吡啶配合物与酵母RNA的相互作用研究

蒋尚达1，王科志*1，刘芙蓉1，张永安1，高丽华2   

  1. (1北京师范大学化学系 北京 100875)
    (2北京工商大学化学与环境工程学院 北京 100037)
  • 投稿日期:2004-08-04 修回日期:2004-12-28 发布日期:2010-12-10
  • 通讯作者: 王科志

Interaction of a Dinuclear Polypyridyl Ruthenium(II) Complex with Yeast-RNA

JIANG Shang-Da1, WANG Ke-Zhi*1, LIU Fu-Rong1, ZHANG Yong-An1, GAO Li-Hua2   

  1. (1 Department of Chemistry, Beijing Normal University, Beijing 100875)
    (2 School of Chemical and Environment Engineering, Beijing Technology and Business University, Beijing 100037)
  • Received:2004-08-04 Revised:2004-12-28 Published:2010-12-10
  • Contact: WANG Ke-Zhi

PDF

897

被引次数

46

用紫外-可见吸收光谱和荧光光谱滴定、稳态荧光淬灭和反向盐滴定实验研究了双核钌(II)配合物[(bpy)2Ru(ebipcH2)Ru(bpy)2](ClO4)4 {bpy＝2,2'-联吡啶; ebipcH2＝N-乙基-4,7-二(咪唑-[4,5-f]-(1,10-邻菲啰啉)-2-基)咔唑}与酵母RNA的相互作用. 结果表明该双核配合物以插入方式与酵母RNA作用, 在生理盐浓度下(≈150 mmol/L NaCl)该配合物与RNA的相互作用明显强于DNA.

关键词: 钌(II)多吡啶配合物, 酵母RNA, 插入作用

The binding of a dinuclear Ru(II) complex of [(bpy)2Ru(ebipcH2)Ru(bpy)2](ClO4)4, where bpy＝2,2'-bipyridine and ebipcH2＝N-ethyl-4,7-bis(imidazolo[4,5-f]-(1,10-phenanthrolin)-2-yl)carbazole, to yeast-RNA has been investigated by UV-visible and emission spectroscopy titrations, steady-state emission quenching and reverse salt titration experiments. The results suggest that the binding of this complex to the RNA be intercalative with a binding constant of 9.1×105 L/mol at 50 mmol/L NaCl, which is much stronger than to the DNA at physiological saline concentration (≈150 mmol/L NaCl).

Key words: ruthenium(II) complex, yeast-RNA, intercalation