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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (9): 833-838.    下一篇

研究论文

无标记DNA在氨基改性导电聚吡咯表面的固定/杂交

张治红，梁燕，闫福丰，闫立军，豆君，李彦山，王力臻，郑先君   

  1. (河南省表界面重点实验室 郑州轻工业学院材料与化工学院 郑州 450002)
  • 投稿日期:2009-10-12 修回日期:2009-11-20 发布日期:2009-12-29
  • 通讯作者: 张治红 E-mail:mainzhh@yahoo.com.cn

Label-free Detection of DNA Immobilization/Hybridization on Conducting Polypyrrole Modified by Amino Groups

Zhang Zhihong* Liang Yan Yan Fufeng Yan Lijun Dou Jun Li Yanshan Wang Lizhen Zheng Xianjun   

  1. (Henan Provincial Key Laboratory of Surface & Interface Science, Zheng Zhou University of Light Industry, Zhengzhou 450002)
  • Received:2009-10-12 Revised:2009-11-20 Published:2009-12-29

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6 | 1

通过吡咯(Py)与其衍生物——6-吡咯己胺(PyHA)的共聚物聚(吡咯-<i>co</i>-6-吡咯己胺)[poly(Py-<i>co</i>-PyHA)]的合成研究, 并采用电化学循环伏安法来考察体系的电化学活性. 在缓冲溶液中, 由于探针DNA链上的负电荷与共聚物分子链上的正电荷之间存在强烈的静电吸引力, 使得DNA能够固定在导电聚合物膜上. 实验结果证明, 目标DNA和聚吡咯薄膜之间不存在非特异性吸附, 而能和探针DNA进行顺利杂交. 此结果为以后研究更为敏感的DNA固定及导电聚合物敏感膜提供了实验基础.

关键词: 导电聚吡咯, 电化学循环伏安法, 无标记DNA固定, DNA杂交

Herein, we synthesized amino functionalized polypyrrole, poly[pyrrole-<i>co</i>-(6-pyrrole-1-yl-

hexylamine)] poly(Py-<i>co</i>-PyHA), to enhance the electrostatic interaction between DNAs and polymer and increase the sensitivity of biosensors. Afterwards, probe DNA was immobilized onto the surface of polypyrrole (PPy) and its copolymer film in PBS which is ascribe to electrostatic adsorption between negative charges of DNA chain and positive charges of copolymer. There is no non-specific adsorption between the target DNA and polymer surface. And the total match target DNA can hybridize with probe DNA onto the polymer. This result will be important to study conducting polymer as potential DNA biosensors in the future.

Key words: conducting pyrrole, electrochemical cyclic voltammetry, label-free DNA immobilization, DNA hybridization