微量热法研究黄连及其主要组分配伍的抑菌作用

化学学报 ›› 2010, Vol. 68 ›› Issue (10): 936-940. 上一篇下一篇

研究论文

微量热法研究黄连及其主要组分配伍的抑菌作用

代春美¹^{,2,3，王伽伯*^{,1，孔维军¹^{,2，肖小河*^{,1，彭成²，赵艳玲¹，金城¹}}}}

(¹解放军第三〇二医院全军中药研究所北京 100039)
(²成都中医药大学药学院成都 610000)
(³辽宁医学院基础学院锦州 121001)

投稿日期:2009-10-26 修回日期:2009-11-17 发布日期:2010-01-05
通讯作者: 代春美 E-mail:springdaidai@163.com

Investigation on the Antibacterial Activity of Coptis chinensis Franch and Its Components Compatibility by Microcalorimetry

Dai Chunmei¹^{,2,3 Wang Jiabo*^{,1 Kong Weijun¹^{,2 Xiao Xiaohe*^{,1 Peng Cheng² Zhao Yanling¹ Jin Cheng¹}}}}

(¹ PLA Institute of Chinese Materia Medica, Hospital of People s Liberation Army, Beijing 100039)
(² Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 610000)
(³ Liaoning Medical University, Jinzhou 121001)

Received:2009-10-26 Revised:2009-11-17 Published:2010-01-05

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摘要/Abstract

基于微量热法, 研究黄连、黄连的主要组分小檗碱、药根碱、巴马汀及其配伍模拟方的抑菌作用. 以HPLC法测定黄连中小檗碱、药根碱和巴马汀的含量, 并根据其含量比值配伍模拟方; 微量热法测定黄连、小檗碱、药根碱、巴马汀及其模拟方对痢疾杆菌的生长代谢曲线, 得出相应的热动力学参数, 并进行对应分析. 结果表明黄连、小檗碱、药根碱、巴马汀及其模拟方对痢疾杆菌的生长代谢均有不同程度的抑制作用, 黄连作用最强, 单体生物碱作用弱, 配伍模拟方作用增强, 但并未显现明显协同作用, 黄连的抑菌作用可能为多种活性成分的综合作用.

关键词: 微量热法, 黄连, 抑菌作用

The antibacterial activity of Coptis chinensis Franch and its key components including berberine, jatrorrhizine, palmatine and compatibility imitation recipes were studied by microcalorimetry. The contents of Ber, Jat and Pal were detected by high-performance liquid chromatography (HPLC) method and the compatibility imitation recipes were prepared according to the content proportion of three kinds of alkaloid in the herb. The power-time curves of Bacillus shigae growth affected by Coptis chinensis Franch, Ber, Jat, Pal and these compatibility imitation recipes were monitored by microcalorimetry. Meanwhile, the biothermo-kinetics parameters obtained from these curves were analyzed using correspondence analysis. Coptis chinensis Franch, Ber, Jat, Pal and compatibility imitation recipes had different inhibitory effect on Bacillus shigae growth. The antibacterial effects of Coptis chinensis Franch and the compatibility imitation recipes were stronger than single alkaloids in this herb, and the compatibility imitation recipes did not showed obvious synergistic effect. It could be concluded that the antibacterial effect of Coptis chinensis Franch was due to the integrated action of its many components.

Key words: microcalorimetry, Coptis chinensis Franch, antibacterial effect

引用此文

代春美, 王伽伯, 孔维军, 肖小河, 彭成, 赵艳玲, 金城. 微量热法研究黄连及其主要组分配伍的抑菌作用[J]. 化学学报, 2010, 68(10): 936-940.

DAI Chun-Mei, WANG Ga-Ba, KONG Wei-Jun, XIAO Xiao-He, BANG Cheng, DIAO Yan-Ling, JIN Cheng. Investigation on the Antibacterial Activity of Coptis chinensis Franch and Its Components Compatibility by Microcalorimetry[J]. Acta Chimica Sinica, 2010, 68(10): 936-940.

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微量热法研究黄连及其主要组分配伍的抑菌作用

Investigation on the Antibacterial Activity of Coptis chinensis Franch and Its Components Compatibility by Microcalorimetry

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