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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (15): 1525-1531. 上一篇    下一篇

研究论文

苹果果胶活性寡聚半乳糖醛酸的分离制备及其ESI-MS分析研究

徐溪，李潇，黄琳娟，王仲孚   

  1. (西北大学生命科学学院 西北大学糖生物学与糖工程研究中心 西安 710069)
  • 投稿日期:2010-03-04 修回日期:2010-04-28 发布日期:2010-05-16
  • 通讯作者: 王仲孚 E-mail:wangzhfnwu@163.com
  • 基金资助:

    国家高技术研究发展计划;国家高技术研究发展计划;教育部新世纪优秀人才支持计划;陕西省重点实验室重点科研计划项目

Studies on Preparation of Oligogalacturonic Acids from Apple Pectin and Analysis by ESI-MS

XU Xi, LI Xiao, HUANG Lin-Juan, WANG Zhong-Fu   

  1. (Glycobiology and Glycotechnology Research Center, Life Science College, Northwest University, Xi an 710069)
  • Received:2010-03-04 Revised:2010-04-28 Published:2010-05-16

PDF

1699

被引次数

16 | 4

苹果果胶多糖经脱酯化得到果胶酸, 以果胶酸为底物通过酶解得到聚合度为2～8的果胶寡糖混合物, 并运用阴离子交换柱层析对果胶寡糖混合物进行了分离制备. 结果表明, 以果胶酸为底物在40 ℃酶解反应1 h为最佳酶解条件得到果胶寡糖混合物, 经分离制备得到聚合度为2～5的寡聚半乳糖醛酸单体, 总得率为37%, TLC和ESI-MS检测其纯度为92%以上. 运用ESI-MS分析寡聚半乳糖醛酸时发现除单体的分子离子峰外, 还存在二聚体的分子离子峰, 并对二聚体的存在进行了确认.

关键词: 苹果果胶, 寡聚半乳糖醛酸, 阴离子交换树脂, 电喷雾质谱

A low degree of esterification pectic acid was obtained by sodium hydroxide treatment of apple pectin. Pectic acid was depolymerized with a commercially available pectinase and a mixture of oligo-GalA (DP2-8) was pooled together, the mixture was separated by a low-pressure anion-exchange resin column chromatography to obtain pure oligo-GalA. The degradation conditions were optimized as enzymatic reaction at 40 ℃ for 1 h, oligo-GalA monomers in the range of 2～5 with a purity degree over 92% analyzed by TLC and ESI-MS were prepared (yield of 37%). The results indicated that there also existed dimer molecular ion peaks along with oligogalacturonic acid monomer molecular ion peaks, and the presence of the dimer were identified by ESI-MS/MS.

Key words: apple pectin, oligogalacturonic acid, anion-exchange resin, ESI-MS