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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (20): 2041-2046. 上一篇    下一篇

研究论文

用于生物检测的链霉亲和素修饰γ-Fe2O3@Au复合颗粒的制备与表征

刘丽赏1，刘洪娜2，李松*,1,2，邓燕1，李小龙1，何农跃*,1,2   

  1. (1湖南工业大学绿色包装与生物纳米技术应用湖南省重点实验室 株洲 412008)
    (2东南大学生物电子国家重点实验室 南京 210096)
  • 投稿日期:2009-12-28 修回日期:2010-05-20 发布日期:2010-06-18
  • 通讯作者: 何农跃 E-mail:nyhe@seu.edu.cn
  • 基金资助:

    自然科学基金;自然科学基金;自然科学基金;863计划;重大科学研究计划;科技重大专项;湖南省自然科学基金;湖南省自然科学基金

Fabrication and Characterization of Streptavidin γ-Fe2O3@Au Nanocomposites for Biological Application

Liu Lishang1 Liu Hongna2 Li Song*,1,2 Deng Yan 1 Li Xiaolong1 He Nongyue*,1,2   

  1. (1 Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412008)
    (2 State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096)
  • Received:2009-12-28 Revised:2010-05-20 Published:2010-06-18

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制备了粒径为30 nm左右的γ-Fe2O3磁性颗粒, 利用巯基硅烷(MPTES)的偶联作用最终制备了粒径约为35.9 nm的金磁复合颗粒. 用X射线粉末衍射仪(XRD)、透射电子显微镜(TEM)、紫外可见吸收光谱(UV-Vis)、傅里叶变换红外光谱(FT-IR)、振动样品磁强计(VSM)等方法对所得金磁颗粒的表面形貌、大小、结构、光学和磁学性质进行了表征. 结果表明: 金成功地包覆到了γ-Fe2O3颗粒的表面, 所得金磁颗粒具有超顺磁性. 利用静电吸附作用, 链霉亲和素有效修饰到了γ-Fe2O3@Au复合磁性颗粒表面. 通过扫描仪检测其捕捉Cy3标记寡核苷酸序列后的荧光信号, 证明了链霉亲和素修饰的γ-Fe2O3@Au复合磁性颗粒有生物活性, 且没有荧光背景, 在生物检测领域表现出了很大的应用前景.

关键词: MPTES (3-Mercaptopropyltriethoxysilane), γ-Fe2O3@Au复合颗粒, 链霉亲和素, 生物检测, 生物电子学

γ-Fe2O3 nanoparticles about 30 nm were prepared using chemical co-precipitation firstly, and then they are modified with thiol group by means of 3-mercaptopropyltriethoxysilane (MPTES), γ-Fe2O3@Au nanoparticles with size of 35.9 nm were obtained. XRD, TEM, UV, FT-IR and VSM analysis showed the surface appearance, size, structure, optical and magnetics property of the gold coated magnetic nanoparticles (γ-Fe2O3@Au). The result showed that Au was coated on the surface of γ-Fe2O3 successfully, the obtained γ-Fe2O3@Au composite nanoparticles are superparamagnetic, streptavidin was modified on the surface of γ-Fe2O3@Au nanoparticles effectively. The fluorescent signal before and after adsorption of Cy3 labeled oligonucleotide showed that the streptavidin modified γ-Fe2O3@Au nanoparticles had good biological activity and without fluorescence background. Most kinds of biological molecular can be combined on the surface of the streptavidin modified γ-Fe2O3@Au nanoparticles through biotin-streptavidin system. Being able to be separated with magnetic field easily, they have great potential application prospects.

Key words: MPTES (3-Mercaptopropyltriethoxysilane), γ-Fe2O3@Au composites, streptavidin, biological application, bioelectronics