纳米SnSe2 空心球上乙酰胆碱酯酶的固定及辛硫磷传感器的构建

doi:10.6023/A1108021

化学学报 ›› 2012, Vol. 70 ›› Issue (08): 1008-1012.DOI: 10.6023/A1108021 上一篇下一篇

研究论文

纳米SnSe₂ 空心球上乙酰胆碱酯酶的固定及辛硫磷传感器的构建

张灿, 张金星, 王坤, 戴志晖

江苏省生物功能材料重点实验室南京师范大学化学与材料科学学院南京 210097

投稿日期:2011-08-02 修回日期:2011-11-25 发布日期:2011-12-14
通讯作者: 戴志晖 E-mail:daizhihuii@njnu.edu.cn
基金资助:
国家自然科学基金(No. 21175069)、教育部新世纪优秀人才支持计划(No. NCET-09-0159)和江苏省优势学科资助项目.

Immobilization of Acetylcholinesterase on SnSe₂ Hollow Spheres for Sensitive Detection of Phoxim

Zhang Can, Zhang Jinxing, Wang Kun, Dai Zhihui

Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097

Received:2011-08-02 Revised:2011-11-25 Published:2011-12-14
Supported by:
Project supported by the National Natural Science Foundation of China (No. 21175069), the Program for New Century Excellent Talents in University (No. NCET-09-0159) and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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摘要/Abstract

描述了一种将乙酰胆碱酯酶固定在SnSe₂ 空心球上检测辛硫磷的简单方法, 用水热法合成了SnSe₂ 空心球, 并用透射电镜对其表征. 固定的乙酰胆碱酯酶能保持其生物学活性, 催化乙酰胆碱为胆碱, 胆碱被氧化产生可检测的信号.基于辛硫磷对乙酰胆碱酯酶活性有抑制作用这个机理, 在理想条件下, 这种传感器对辛硫磷检测的线性范围是0.008～56 μg/mL, 检测限为0.004 μg/mL. 这种新型的传感器有很好的稳定性和重现性. 这项工作表明SnSe₂ 空心球可以作为固定乙酰胆碱酯酶的理想载体并用于构建相应的传感器.

关键词: 乙酰胆碱酯酶, SnSe₂ 空心球, 辛硫磷

A simple method for immobilization of acetylcholinesterase (AChE) on SnSe₂ hollow spheres for detection of phoxim was described. SnSe₂ hollow spheres were prepared by hydrothermal methods and were characterized by TEM. The immobilized AChE retained its biological activity well and could catalyze the hydrolysis of acetylthiocholine to form thiocholine, which was then oxidized to produce detectable signal. Based on the inhibition toward the enzymatic activity of AChE by phoxim, under optimal conditions, the sensors could be used for the determination of phoxim ranging from 0.008 to 56 μg/mL with the detection limit of 0.004 μg/mL. The developed phoxim biosensors exhibited good stability and reproducibility. This work demonstrated that SnSe₂ hollow sphere could be served as an ideal carrier for immobilization of AChE to fabricate corresponding biosensor.

Key words: acetylcholinesterase, SnSe₂ hollow spheres, phoxim

引用此文

张灿, 张金星, 王坤, 戴志晖. 纳米SnSe₂ 空心球上乙酰胆碱酯酶的固定及辛硫磷传感器的构建[J]. 化学学报, 2012, 70(08): 1008-1012.

Zhang Can, Zhang Jinxing, Wang Kun, Dai Zhihui. Immobilization of Acetylcholinesterase on SnSe₂ Hollow Spheres for Sensitive Detection of Phoxim[J]. Acta Chimica Sinica, 2012, 70(08): 1008-1012.

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参考文献

1 Arduini, F.; Ricci, F.; Tuta, C. S.; Moscone, D.; Amine, A.; Palleschi, G. Anal. Chim. Acta 2006, 580, 155.

2 Li, B. X.; He, Y. Z.; Xu, C. L. Talanta 2007, 72, 223.

3 Garbin, J. R.; Milori, D. M. B. P.; Simoes, M. L.; Silva, W. T. L.; Neto, L. M. Chemosphere 2007, 66, 1692.

4 Khanmohammadia, M.; Karimi, M. A.; Ghasemi, K.; Jabbari, M.; Garmarudi, A. B. Talanta 2007, 72, 620.

5 Fytianos, K.; Raikos, N.; Theodoridis, G.; Velinova, Z.; Tsoukali, H. Chemosphere 2006, 65, 2090.

6 Kim, T. H.; Kuca, K.; Jun, D.; Jung, Y. K. Bioorg. Med. Chem. Lett. 2005, 15, 2914.

7 López, F. J.; Pitarch, E.; Egea, S.; Beltran, J.; Hernández, F. Anal. Chim. Acta 2001, 433, 217.

8 Corcia, A.; Marcheti, M. Anal. Chem. 1991, 63, 580.

9 Futagami, K.; Narazaki, C.; Kataoka, Y.; Shuto, H.; Oishi, R. J. Chromatogr. B 1997, 704, 369.

10 Schellin, M.; Hauser, B.; Popp, P. J. Chromatogr. A 2004,1040, 25.

11 Sun, X.; Wang, X. Y. Biosens. Bioelectron. 2010, 25, 2611.

12 Gong, J. M.; Miao, X. J.; Zhou, T.; Zhang, L. Z. Talanta2011, 85, 1344.

13 Shi, M. H.; Xu, J. J.; Zhang, S.; Liu, B. H.; Kong, J. L. Talanta 2006, 68, 1089.

14 Massoulie, J.; Pezzementi, L.; Bon, S.; Krejci, E.; Vallette, F. M. Prog. Neurobiol. 1993, 41, 31.

15 Shulga, O.; Kirchhoff, J. R. Electrochem. Commun. 2007, 9,935.

16 Liu, G. D.; Lin, Y. H. Anal. Chem. 2005, 77, 5894.

17 Chauhan, N.; Pundir, C. S. Anal. Chim. Acta 2011, 701, 66.

18 Du, D.; Ding, J. W.; Cai, J.; Zhang, A. D. Colloids Surf. B2007, 58, 145.

19 Du, D.; Chen, S. Z.; Cai, J.; Zhang, A. D. Biosens. Bioelectron.2007, 23, 130.

20 Du, D.; Chen, S. Z.; Cai, J.; Song, D. D. J. Electroanal. Chem. 2007, 611, 60.

21 Kandimalla, V. B.; Ju, H. X. Chem. Eur. J. 2006, 12, 1074.

22 Du, D.; Huang, X.; Cai, J.; Zhang, A. D. Sens. Actuators, A2007, 127, 531.

23 Du, D.; Chen, S. Z.; Cai, J.; Zhang, A. D. Talanta 2008, 74,766.

24 Wang, W. Z.; Geng, Y.; Qian, Y. T.; Wang, C.; Liu, X. M. Mater. Res. Bull. 1999, 34, 403.

25 Shen, J.; Blachnik, R. Thermochim. Acta 2003, 399, 245.

26 Subramanian, B.; Sanjeeviraja, C.; Jayachandran, M. J. Cryst. Growth 2002, 234, 421.

27 Achimovicova, M.; da Silva, K. L.; Daneu, N.; Recnik, A.; Indris, S.; Hain, H.; Scheuermann, M.; Hahn, H.; Sepelak, V. J. Mater. Chem. 2011, 21, 5873.

28 Amalraj, L.; Jayachandran, M.; Sanjeeviraja, C. Mater. Res. Bull. 2004, 39, 14.

29 Peng, H. R.; Huang, J. J. Qingdao Univ. Sci. Technol. 2006,27, 427 (in Chinese). (彭红瑞, 黄劲, 青岛科技大学学报, 2006, 27, 427.)

30 Du, D.; Ding, J. W.; Cai, J.; Zhang, A. D. J. Electroanal. Chem. 2007, 605, 53.

31 Gong, J. M.; Wang, L. Y.; Zhang, L. Z. Biosens. Bioelectron.2009, 24, 2285.

32 Li, X. H.; Xie, Z.; Min, H.; Li, C.; Liu, M.; Xian, Y.; Jin, L. Electroanalysis 2006, 18, 2163.

33 Du, D.; Huang, X.; Cai, J.; Zhang, A. D. Biosens. Bioelectron.2007, 23, 285.

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纳米SnSe₂ 空心球上乙酰胆碱酯酶的固定及辛硫磷传感器的构建

Immobilization of Acetylcholinesterase on SnSe₂ Hollow Spheres for Sensitive Detection of Phoxim

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