免疫金铂纳米合金催化共振散射光谱法测定痕量人绒毛膜促性腺激素

doi:10.6023/A12030074

化学学报 ›› 2012, Vol. 70 ›› Issue (16): 1748-1754.DOI: 10.6023/A12030074 上一篇

研究论文

免疫金铂纳米合金催化共振散射光谱法测定痕量人绒毛膜促性腺激素

蒋治良, 姚东梅, 李芳, 梁爱惠

药用资源化学与药物分子工程教育部重点实验室珍稀濒危动植物生态与环境保护省部共建教育部重点实验室广西师范大学桂林 541004

投稿日期:2012-03-30 发布日期:2012-05-26
通讯作者: 蒋治良, 梁爱惠
基金资助:
项目受国家自然科学基金(Nos. 21165005, 21075023)和药用资源化学与药物分子工程教育部重点实验室基金(No. CMEMR2011-10)资助.

Resonance Scattering Assay for Trace Human Chorionic Gonadotrophin Using Gold-platinum Nanoalloy Immunoprobe as Catalyst

Jiang Zhiliang, Yao Dongmei, Li Fang, Liang Aihui

Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Education Ministry;Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry,Guangxi Normal University, Guilin 541004

Received:2012-03-30 Published:2012-05-26
Supported by:
Project supported by the National Natural Science Foundation of China (Nos. 21165005, 21075023) and the Fund of Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University), Ministry of Education of China (No. CMEMR2011-10).

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摘要/Abstract

以NaBH₄为还原剂, 制备了金与铂物质的量比为49∶1的金铂纳米合金(GP). 用兔抗人绒毛膜促性腺激素抗体(RhCG)修饰AuPt获得了免疫纳米合金探针(GP-RhCG). 在pH 5.8磷酸氢二钠-柠檬酸缓冲溶液及KCl存在的条件下, GP-RhCG探针发生非特异性聚集, 在590 nm处有一个较强的共振散射峰. 当有人绒毛膜促性腺激素(hCG)存在时, 聚集的GP-RhCG探针与hCG发生特异性结合, 生成分散性较好的GP-RhCG-hCG免疫复合物, 导致590 nm处共振散射峰强度降低. 其共振散射峰强度降低值ΔI_{590 nm}与hCG浓度在6.67～86.7 ng/mL范围内呈现良好线性关系. 免疫反应液中形成的GP-RhCG-hCG免疫复合物对葡萄糖-铜(II)体系具有较强的催化作用, 其产物在610 nm处有一较强共振散射峰. 随着hCG浓度增大, 形成的GP-RhCG-hCG复合物越多, 其催化作用增强, 610 nm处的共振散射峰增强. 其共振散射峰增大值ΔI_{610 nm}与hCG浓度在3.33～133 ng/mL范围内呈线性关系.

关键词: 人绒毛膜促性腺激素, 金铂纳米合金, 免疫纳米催化, 共振散射光谱法

In the presence of sodium citrate as stabilizer, a gold-platinum nanoalloy (GP) in the mole ratio of 49∶1 was prepared by using NaBH₄ as a reducing agent. The rabbit anti-human chorionic gonadotrophin antibody (RhCG) was labeled by the GP to obtain a stable immune GP probe (GP-RhCG) for the human chorionic gonadotrophin (hCG). In a pH 5.8 NaH₂PO₄-citric acid buffer solution and in the presence of KCl, the GP-RhCG probe was aggregated un-specifically to big particles that exhibited a resonance scattering (RS) peak at 590 nm. When the hCG was added, the GP-RhCG probe combined specifically with the hCG to form a dispersed GP-RhCG-hCG immunocomplex that resulted in the RS intensity decreasing at 590 nm. The decreased RS intensity (ΔI_{590 nm}) is linear with hCG concentration in the range of 6.67—86.7 ng/mL, with a detection limit of 3.7 ng/mL hCG. The immunocomplex exhibited a strong catalytic effect on the slow Cu₂O particle reaction of glucose-Cu(II), and the particles appeared a RS peak at 610 nm. When the hCG concentration increased in the immune reaction system, the immunocomplex concentration increased, and the catalytic product of Cu₂O particles increased, which caused the enhancement of RS intensity at 610 nm. The increased intensity (ΔI_{610 nm}) is linear with the hCG concentration in the range of 3.33—133 ng/mL, with a detection limit of 1.7 ng/mL hCG. In addition, the influence of foreign substances on the determination of hCG was examined. The results show that 150 times of L-aspartic acid, urea, BSA, glycine, Ca(II) and Zn(II), 50 times of L-lysine and L-cysteine, 30 times of IgG and IgA, 40 times of IgM did not interfered with the determination of 80 ng/mL hCG. This indicates that this method has good selectivity. The hCG content in the serum sample was analyzed by the GP RS method and GP-catalytic RS method, the results are agreement with those of the chemiluminescence experiments. Both RS methods are of some advantages including low-cost, simplicity, rapidity, high sensitivity and selectivity.

Key words: human chorionic gonadotrophin, gold-platinum nanoalloy, immunonanocatalysis, resonance scattering assay

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蒋治良, 姚东梅, 李芳, 梁爱惠. 免疫金铂纳米合金催化共振散射光谱法测定痕量人绒毛膜促性腺激素[J]. 化学学报, 2012, 70(16): 1748-1754.

Jiang Zhiliang, Yao Dongmei, Li Fang, Liang Aihui. Resonance Scattering Assay for Trace Human Chorionic Gonadotrophin Using Gold-platinum Nanoalloy Immunoprobe as Catalyst[J]. Acta Chimica Sinica, 2012, 70(16): 1748-1754.

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参考文献

[1] Sanvicens, N.; Marco, M. P. Trends Biotechnol. 2008, 26, 425.

[2] Tongprasert, F.; Wanapirak, C.; Tongsong, T. Prenatal Diagn. 2011, 31, 430.

[3] Cole, L. A.; Butler, S. A.; Khanlian, S. A.; Giddings, A.; Muller, C. Y.; Seckl, M. J.; Kohorn, E. I. Gynecol. Oncol. 2006, 102, 151.

[4] Yang, H.-C.; Yuan, R.; Chai, Y.-Q.; Zhuo, Y. Colloids Surf., B 2011, 82, 463.

[5] Chai, R.; Yuan, R.; Chai, Y.-Q.; Ou, C.-F.; Cao, S.-R.; Li, X.-L. Talanta 2008, 74, 1330.

[6] Lim, T. K.; Matsunaga, T. Biosens. Bioelectron. 2001, 16, 1063.

[7] Yang, G.-M.; Yang, X.-Y.; Yang, C.-Y.; Yang, Y.-H. Colloids Surf., A 2011, 389, 195.

[8] Mao, L.; Yuan, R.; Chai, Y.-Q.; Zhuo, Y.; Yang, X. Sens. Actuators B 2010, 149, 226.

[9] Li, R.; Wu, D.; Li, H.; Xu, C.-X.; Wang, H.; Zhao, Y.-F.; Cai, Y.-Y.; Wei, Q.; Du, B. Anal. Biochem. 2011, 414, 196.

[10] Kim, H. S.; Pyun, J. C. Procedia Chem. 2009, 1, 1043.

[11] Luo, L.; Zhang, Z.-J.; Hou, L.-Y.; Wang, J.-L.; Tian, W. Talanta 2007, 72, 1293.

[12] Yan, X.; Huang, Z.-B.; He, M.; Liao, X.-M.; Zhang, C.-Q.; Yin, G.-F.; Gu, J.-W. Colloids Surf., B 2012, 89, 86.

[13] Nakamura, N.; Lim, T. K.; Jeong, J. M.; Matsunaga, T. Anal. Chim. Acta 2001, 439, 125.

[14] Hudelist, G.; Wuelfing, P.; Czerwenka, K.; Knöfler, M.; Haider, S.; Fink-Retter, A.; Gschwantler-Kaulich, D.; Pfeiler, G.; Kubista, E.; Singer, C. F. J. Cancer Res. Clin. Oncol. 2009, 135, 191.

[15] Jiang, Z.-L.; Zou, M.-J.; Liang, A.-H. Clin. Chim. Acta 2008, 387, 24.

[16] Liang, A.-H.; Zou, M.-J.; Jiang, Z.-L. Talanta 2008, 75, 1214.

[17] Nakamura, K.; Satomura, S.; Matsuura, S. Anal. Chem. 1993, 65, 613.

[18] Lv, M.-X.; Zhang, J.; Shen, Q.-Y.; Chen, K.-M.; Feng, G.-Y. Chin. J. Biomed. Eng. 1992, 11, 174. (吕鸣详, 张杰, 申群英, 陈昆明, 冯光易, 中国生物医学工程学报, 1992, 11, 174.)

[19] Robinson, G. A.; Hill, H. A.; Philo, R. D.; Gear, J. M.; Rattle, S. J.; Forrest, G. C. Clin. Chem. 1985, 31, 1449.

[20] Guo, X.-Y.; Guan, Y.-P.; Yang, B.; Wang, Y.-N.; Lan, H.-L.; Yang, Z.-H.; Lu, Z.-H. Int. J. Mol. Sci. 2006, 7, 274.

[21] Vaitukatitis, J. L.; Braunstein, G. D.; Ross, G. T. Am. J. Obstet. Gynecol. 1972, 113, 751.

[22] Tung, N. H.; Chikae, M.; Ukita, Y.; Viet, P. H.; Takamura, Y. Anal. Chem. 2012, 84, 1210.

[23] Liang, A.-H.; Huang, Y.-J.; Jiang, Z.-L. Clin. Chim. Acta 2007, 383, 73.

[24] Lin, S.-M.; Fan, Y.-Y.; Jiang, Z.-L.; Wen, G.-Q.; Liu, Q.-Y.; Liang, A.-H. Acta Chim. Sinica 2010, 68, 339. (凌绍明, 范燕燕, 蒋治良, 温桂清, 刘庆业, 梁爱惠, 化学学报, 2010, 68, 339.)

[25] Jiang, Z.-L.; Sun, S.-J.; Liang, A.-H.; Huang, W.-X.; Qin, A.-M. Clin. Chem. 2006, 52, 1389.

[26] Li, J.-F.; Jiang, Z.-L.; Deng, A.-P. Chin. J. Chem. 2008, 26, 2297.

[27] Jiang, Z.-L.; Fan, Y.-Y.; Liang, A.-H.; Wen, G.-Q.; Liu, Q.-Y.; Li, T.-S. Plasmonics 2010, 5, 375.

[28] Jiang, C.-N.; Liang, A.-H.; Jiang, Z.-L. Acta Chim. Sinica 2011, 69, 714. (蒋彩娜, 梁爱惠, 蒋治良, 化学学报, 2011, 69, 714.)

[29] Zhang, Y.; Liang, A.-H.; Zhou, L.-P.; Qin, H.-M.; Ouyang, H.-X.; Wang, P.-F.; Jiang, Z.-L. Acta Chim. Sinica 2011, 69, 2153. (张轶, 梁爱惠, 周莲平, 覃惠敏, 欧阳辉祥, 王鹏飞, 蒋治良, 化学学报, 2011, 69, 2153.)

[30] Jiang, Z.-L.; Huang, Y.-J.; Liang, A.-H.; Pan, H.-C.; Liu, Q.-Y. Biosens. Bioelectron. 2009, 24, 1674. Jiang, Z.-L.; Liao, X.-J.; Deng, A.-P.; Liang, A.-H.; Li, J.-S.; Pan, H.-C.; Li, J.-F.; Wang, S.-M.; Huang, Y.-J. Anal. Chem. 2008, 80, 8681.

免疫金铂纳米合金催化共振散射光谱法测定痕量人绒毛膜促性腺激素

Resonance Scattering Assay for Trace Human Chorionic Gonadotrophin Using Gold-platinum Nanoalloy Immunoprobe as Catalyst

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