化学学报 ›› 2014, Vol. 72 ›› Issue (1): 51-55.DOI: 10.6023/A13090943 上一篇    下一篇

研究论文

紫外光辐照制备超亲水再生丝素蛋白膜

尹建伟, 段郁, 邵正中   

  1. 聚合物分子工程国家重点实验室 复旦大学高分子科学系和先进材料实验室 上海 200433
  • 收稿日期:2013-09-09 出版日期:2014-01-14 发布日期:2013-11-14
  • 通讯作者: 邵正中,E-mail:zzshao@fudan.edu.cn E-mail:zzshao@fudan.edu.cn
  • 基金资助:

    项目受国家自然科学基金委重点项目(No. 21034003)和上海市优秀学术带头人计划(No. 12XD1401000)资助.

UV Induced Superhydrophilicity of RSF Film

Yin Jianwei, Duan Yu, Shao Zhengzhong   

  1. State Key Laboratory of Molecular Engineering of Polymers, Laboratory of Advanced Materials and Department of Macromolecular Science, Fudan University, Shanghai 200433
  • Received:2013-09-09 Online:2014-01-14 Published:2013-11-14
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 21034003) and the Program of Shanghai Subject Chief Scientist (No. 12XD1401000).

众多的研究已表明生物材料的表面润湿性(wettability)对于细胞的粘附有重要的影响. 在通过浇铸成膜并辅以乙醇后处理方法制备非水溶性再生丝素蛋白膜的基础上,我们采用紫外光辐照的方法以改变此类丝素蛋白膜的表面特性. 接触角测试的结果显示,随着紫外光照时间的增加,丝素蛋白膜表面与水的接触角逐渐降低,最终可以达到超亲水的状态;表面X射线光电子能谱(XPS)分析表明,这是由于丝素蛋白膜表面亲水性基团(包括羧基和羰基等)的含量随着紫外光照时间的延长而增加所致. 同时我们发现,经紫外光辐照一定时间后,再生丝素蛋白膜的力学性能并没有发生显著的降低. 显然,此类具有较高力学性能且表面亲水性能较好的再生丝素蛋白膜有可能被进一步应用于生物医学领域.

关键词: 再生丝素蛋白膜, 超亲水, 紫外光照, X射线光电子能谱, 力学性能

The research has indicated that the surface wettability of biological material has important effects on the cell attachment. The water insoluble regenerated silk fibroin film, which was made through solution-casting and then treated using ethanol was treated by UV light in order to change its surface properties. The effects of the UV irradiation on the surface wettability of RSF film were investigated. The results showed that UV irradiation induced the superhydrophilic of the surface of RSF film from non-hydrophilic, as the water contact angle of the RSF film was changed from 89°to less than 10°. However, such superhydrophilicity is time-dependent in a certain degree, because the water contact angle of the irradiated RSF film was increased to around 25° during storage of the film. XPS indicated that the increase of the hydrophilic group on surface from 24.1% to 40.5% is the major reason. Furthermore, the tensile testing demonstrated that UV irradiation has little effect on the mechanical properties of bulk RSF based materials. In general, the UV irradiated RSF film with hydrophilic surface and significant mechanical properties may be a promising natural material which has widely potential application in the biomedical field.

Key words: regenerated silk fibroin film, superhydrophilic, UV irradiation, XPS, mechanical property