Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (08): 1008-1012.DOI: 10.6023/A1108021 Previous Articles     Next Articles

Full Papers

纳米SnSe2 空心球上乙酰胆碱酯酶的固定及辛硫磷传感器的构建

张灿, 张金星, 王坤, 戴志晖   

  1. 江苏省生物功能材料重点实验室 南京师范大学化学与材料科学学院 南京 210097
  • 投稿日期:2011-08-02 修回日期:2011-11-25 发布日期:2011-12-14
  • 通讯作者: 戴志晖
  • 基金资助:

    国家自然科学基金(No. 21175069)、教育部新世纪优秀人才支持计划(No. NCET-09-0159)和江苏省优势学科资助项目.

Immobilization of Acetylcholinesterase on SnSe2 Hollow Spheres for Sensitive Detection of Phoxim

Zhang Can, Zhang Jinxing, Wang Kun, Dai Zhihui   

  1. Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097
  • Received:2011-08-02 Revised:2011-11-25 Published:2011-12-14
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 21175069), the Program for New Century Excellent Talents in University (No. NCET-09-0159) and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

A simple method for immobilization of acetylcholinesterase (AChE) on SnSe2 hollow spheres for detection of phoxim was described. SnSe2 hollow spheres were prepared by hydrothermal methods and were characterized by TEM. The immobilized AChE retained its biological activity well and could catalyze the hydrolysis of acetylthiocholine to form thiocholine, which was then oxidized to produce detectable signal. Based on the inhibition toward the enzymatic activity of AChE by phoxim, under optimal conditions, the sensors could be used for the determination of phoxim ranging from 0.008 to 56 μg/mL with the detection limit of 0.004 μg/mL. The developed phoxim biosensors exhibited good stability and reproducibility. This work demonstrated that SnSe2 hollow sphere could be served as an ideal carrier for immobilization of AChE to fabricate corresponding biosensor.

Key words: acetylcholinesterase, SnSe2 hollow spheres, phoxim