Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (10): 1153-1158.DOI: 10.6023/A1111253 Previous Articles     Next Articles

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肖飞, 白静, 张苧丹, 赖彦君, 金利通   

  1. 华东师范大学化学系 上海 200062
  • 投稿日期:2011-11-25 修回日期:2012-02-27 发布日期:2012-05-28
  • 通讯作者: 金利通
  • 基金资助:

    上海市科委专项(No. 06dz05824)资助项目.

Construction of Dual Signal Amplification Competitive Immunosensor for Detection of Clenbuterol

Xiao Fei, Bai Jing, Zhang Ningdan, Lai Yanjun, Jin Litong   

  1. Department of Chemistry, East China Normal University, Shanghai 200062
  • Received:2011-11-25 Revised:2012-02-27 Published:2012-05-28
  • Supported by:

    Project supported by Shanghai Science and Technology Commission Foundation (No. 06dz05824).

A dual signal amplification immunosensor based on glucose oxidase (GOD)-functionalized nanocomposites was developed for the detection of clenbuterol. The characteristics of the nanocomposites were confirmed by scanning electron microscopy (SEM) and spectrophotometry. The results indicate that GOD in the nanocomposites keeps good enzyme dynamic response and electro-catalysis performance. The detection mechanism of the immunosensor was investigated by electrochemical methods of differential pulse voltammetry (DPV). A set of experimental conditions were also optimized for the detection of clenbuterol. The developed immunosensor exhibited high sensitivity, selectivity and reproducibility, which had a wide linear range of 0.01~100 ng/mL and a low detection limit of 4.50 pg/mL for clenbuterol. Accurate detection of clenbuterol in pig liver samples was demonstrated by standard addition method.

Key words: glucose oxidase, immunosensor, clenbuterol, β-agonist