Preparation and Bioelectrochemical Application of Gold Nanoparticles-Chitosan-Graphene Nanomaterials

doi:10.6023/A12060284

Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (21): 2213-2219.DOI: 10.6023/A12060284 Previous Articles Next Articles

Article

金纳米粒子-壳聚糖-石墨烯纳米复合材料的制备及其在生物电化学中的应用

张谦^a,b, 吴抒遥^a,b, 何茂伟^a,b, 张玲^c, 刘洋^d, 李景虹^d, 宋溪明^a,b

a 辽宁大学绿色合成与先进材料制备化学辽宁省重点实验室沈阳 110036;
b 辽宁大学化学院沈阳 110036;
c 沈阳师范大学化学与生命科学学院沈阳 110034;
d 清华大学化学系北京 100084

投稿日期:2012-06-06 发布日期:2012-09-17
通讯作者: 李景虹, 宋溪明 E-mail:songlab@lnu.edu.cn, jhli@mail.tsinghua.edu.cn
基金资助:
项目受国家自然科学基金(Nos. 21071070, 21005046, 20901035, 51273087, 21235004, 21203126)、辽宁省高等学校创新团队基金(No. LT2011001)、辽宁省自然科学基金(No. 201202088)、2013年辽宁大学青年科研基金和辽宁大学创新人才培养基金资助.

Preparation and Bioelectrochemical Application of Gold Nanoparticles-Chitosan-Graphene Nanomaterials

Zhang Qian^a,b, Wu Shuyao^a,b, He Maowei^a,b, Zhang Ling^c, Liu Yang^d, Li Jinghong^d, Song Xi-Ming^a,b

a Liaoning Provincial Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials, Liaoning University, Shenyang 110036;
b College of Chemistry, Liaoning University, Shenyang 110036;
c College of Chemistry and Life Science, Shenyang Normal University, Shenyang 110034;
d Department of Chemistry, Tsinghua University, Beijing 100084

Received:2012-06-06 Published:2012-09-17
Supported by:
Project supported by the National Natural Science Foundation of China (Nos. 21071070, 21005046, 20901035, 51273087, 21235004 and 21203126), the Program for Liaoning Innovative Research Team in University (No. LT2011001), Natural Science Foundation of Liaoning Province (No. 201202088), the Foundation for Young Scholars of Liaoning University (2013) and the Foundation of 211 Project for Innovative Talents Training, Liaoning University.

1. Preparation and Bioelectrochemical Application of Gold Nanoparticles-Chitosan-Graphene Nanomaterials.PDF(113KB)

Abstract

Based on the covalent modification and in-situ reduction, chitosan and gold nanoparticles were integrated with graphene to form a novel gold nanoparticles/chitosan-graphene (AuNPs/Chit-GP) nanocomposite. The process of the fabrication of AuNPs/Chit-GP nanocomposite consisted of the following steps. Firstly, carboxyl-GP was prepared based on the reduction and modification of graphene oxide (GO). Thereafter, chitosan was covalently functionalized onto graphene nanosheet to fabricate Chit-GP. The AuNPs/Chit-GP nanocomposite was finally synthesized by the in-situ reduction of HAuCl₄in the presence of Chit-GP. Fourier transform infrared (FT-IR) spectra, ultraviolet-visible (UV-vis) absorption spectra, transmission electron microscopy (TEM) and X-ray diffraction (XRD) were utilized to characterize structure and morphology of the as synthesized nanocomposite. Because of the surface functionalization of chitosan, the composite exhibited positive charge. In that case, the negatively charged glucose oxidase (GOD) could further immobilize onto AuNPs/Chit-GP via electrostatic interaction under mild experimental condition. With the advantages of graphene, Au nanoparticles and chitosan, AuNPs/Chit-GP can offer a conductive and favorable microenvironment for the immobilized GOD to achieve direct electrochemistry. The direct electron transfer (DET) reaction of the immoblized GOD was studied by cyclic voltammetry in 0.1 mol/L phosphate buffer solution (PBS, pH 7.4). A pair of well-defined, quasi-reversible redox peaks of GOD were obtained at GOD/AuNPs/Chit-GP/GC modified electrode, with a formal potential (vs. Ag/AgCl) being -0.44 V. Moreover, the as fabricated GOD/AuNPs/Chit-GP/GC modified electrode exhibited excellent catalytic performance towards glucose. The electrocatalytic response of GOD/AuNPs/Chit-GP/GC modified electrode altered linearly with the glucose concentration ranging from 2.1 to 5.7 μmol/L. The detection limit and the sensitivity of the enzyme electrode were 0.7 μmol/L (S/N＝3) and 79.71 mA·cm^-2·mM^-1, respectively. Because of the biocompability of the AuNPs/Chit-GP, GOD/AuNPs/Chit-GP/GC modified electrode also exhibited acceptable reproducibility and excellent stability. Therefore, such a novel nanocomposite composed of metal nanoparticles, biocompatible macromolecules and graphene provides an efficient platform for the development of mediator-free electrochemical biosensors.

Key words: gold nanoparticles, chitosan, graphene, glucose oxidase, direct electrochemistry

Cite this article

Zhang Qian, Wu Shuyao, He Maowei, Zhang Ling, Liu Yang, Li Jinghong, Song Xi-Ming. Preparation and Bioelectrochemical Application of Gold Nanoparticles-Chitosan-Graphene Nanomaterials[J]. Acta Chimica Sinica, 2012, 70(21): 2213-2219.

Export EndNote|Reference Manager|ProCite|BibTeX|RefWorks

share this article

References

[1] Wang, Y.; Li, Z. H.; Li, J. H.; Lin, Y. H. Trends Biotechnol. 2011, 29, 205.
[2] Liu, Y.; Wang, M.; Zhao, F.; Xu, Z.; Dong, S. J. Biosens. Bioelectron. 2005, 21, 984.
[3] Zeng, Q.; Cheng, J. S.; Tang, L. H.; Liu, X. F.; Liu, Y. Z.; Li, J. H.; Jiang, J. H. Adv. Funct. Mater. 2010, 20, 3366.
[4] Zhang, L.; Zhang, Q.; Li, J. H. Adv. Funct. Mater. 2007, 17, 1958.
[5] Zhang, Y.; Zheng, J. b. Acta Chim. Sinica 2011, 69, 1903. (张亚, 郑建斌, 化学学报, 2011, 69, 1903.)
[6] Wang, Y.; Li, Z.; Hu, D.; Lin, C. T.; Li, J. H.; Lin, Y. H. J. Am. Chem. Soc. 2010, 132, 9274.
[7] Tang, L. H.; Wang, Y.; Li, Y. M.; Feng, H. B.; Lu, J.; Li, J. H. Adv. Funct. Mater. 2009, 19, 782.
[8] Wang, Y.; Shao, Y. Y.; Dean W. M.; Lin, Y. H.; Li, J. H. ACS Nano 2010, 4, 1790.
[9] Liu, S.; Tian, J. Q.; Luo, Y. L.; Lu, W. B.; Sun, X. P. Biosens. Bioelectron. 2011, 26, 4491.
[10] Jiang, H. J.; Zhao, Y.; Yang, H.; Aking, D. L. Mater. Chem. Phys. 2009, 114, 879.
[11] Chen, D.; Tang, L. H.; Li, J. H. Chem. Soc. Rev. 2010, 39, 3157.
[12] Zhang, H.; Lv, X. J.; Li, Y. M.; Wang, Y.; Li, J. H. ACS Nano 2009, 4, 380.
[13] Jin, Y.; Chen, H.-Y.; Chen, M.-H.; Liu, N.; Li, Q.-W. Acta Phys.-Chim. Sinica 2012, 28, 609. (靳瑜, 陈宏源, 陈名海, 刘宁, 李清文, 物理化学学报, 2012, 28, 609.)
[14] Wu, J. F.; Xu, M. Q.; Zhao, G. C. Electrochem. Commun. 2010, 12, 175.
[15] Swoboda, B. E. P.; Massey, V. J. Biol. Chem. 1965, 240, 2209.
[16] Leskovac, V.; Trivić, S.; Wohlfahrt, G.; Kandrac, J.; Pericin, D. Int. J. Biochem. Cell B 2005, 37, 731.
[17] Dupont, J.; Spencer, J. Angew. Chem. Int. Ed. 2004, 43, 5296.
[18] Shan, C.; Yang, H.; Song, J.; Han, D.; Ivaska, A.; Niu, L. Anal. Chem. 2009, 81, 2378.
[19] Liu, Q.; Lu, X. B.; Li, J.; Yao, X.; Li, J. H. Biosens. Bioelectron. 2007, 22, 3203.
[20] Xiao, C.; Chu, X.; Wu, B.; Pang, H.; Zhang, X.; Chen, J. Talanta 2010, 80, 1719.
[21] Liu, S. Q.; Ju, H. X. Biosens. Bioelectron. 2003, 19, 177.
[22] Kang, X. H.; Wang, J.; Wu, H.; Aksay, I. A.; Liu, J.; Lin, Y. H. Biosens. Bioelectron. 2009, 25, 901.
[23] Hummers, W. S.; Offeman, R. E. J. Am. Chem. Soc. 1958, 80, 1339. Lomeda, J. R.; Doyle, C. D.; Kosynkin, D. V.; Hwang, W. F.; Tour, J. M. J. Am. Chem. Soc. 2008, 130, 16201.

金纳米粒子-壳聚糖-石墨烯纳米复合材料的制备及其在生物电化学中的应用

Preparation and Bioelectrochemical Application of Gold Nanoparticles-Chitosan-Graphene Nanomaterials

PDF

Supporting Info.

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Congcong Ning, Qian Yang, Amin Mao, Zijia Tang, Yan Jin, Baoshan Hu. Research Progress in Controllable Preparation of Graphene Nanoribbons [J]. Acta Chimica Sinica, 2023, 81(4): 406-419.
[2]	Wen Liu, Yujie Wang, Huiqin Yang, Chengjie Li, Na Wu, Yang Yan. The Preparation of Carbon Nanotubes/Reduced Graphene Oxide Current Collector by Non-covalent Induction of Ionic Liquid for Sodium Metal Anode [J]. Acta Chimica Sinica, 2023, 81(10): 1379-1386.
[3]	Shaobing Yan, Long Jiao, Chuanxin He, Hailong Jiang. Pyrolysis of ZIF-67/Graphene Composite to Co Nanoparticles Confined in N-Doped Carbon for Efficient Electrocatalytic Oxygen Reduction [J]. Acta Chimica Sinica, 2022, 80(8): 1084-1090.
[4]	Xusheng Wang, Xu Yang, Chunhui Chen, Hongfang Li, Yuanbiao Huang, Rong Cao. Graphene Quantum Dots Supported on Fe-based Metal-Organic Frameworks for Efficient Photocatalytic CO₂ Reduction^※ [J]. Acta Chimica Sinica, 2022, 80(1): 22-28.
[5]	Yao Zhai, Guoxiang Xin, Jiaqi Wang, Bangwen Zhang, Jinling Song, Xiaoxu Liu. Microwave-assisted Synthesis of rGO/CeO₂ Supercapacitor Electrode Materials with Excellent Electrochemical Properties [J]. Acta Chimica Sinica, 2021, 79(9): 1129-1137.
[6]	Chang-An Liu, Shi-Bo Hong, Bei Li. Molecular Dynamics Simulation of the Stability Behavior of Graphene in Glycerol/Urea Solvents in Liquid-Phase Exfoliation [J]. Acta Chimica Sinica, 2021, 79(4): 530-538.
[7]	Jie Huang, Jiangbo Xi, Wei Chen, Zhengwu Bai. Graphene-derived Materials for Metal-free Carbocatalysis of Organic Reactions [J]. Acta Chimica Sinica, 2021, 79(11): 1360-1371.
[8]	Hua Yue, Guanghui Ma. Advances in Functionalized Carriers Based on Graphene's Unique Biological Interface Effect [J]. Acta Chimica Sinica, 2021, 79(10): 1244-1256.
[9]	Yanyu Yang, Xing Wang, Decheng Wu. Chitosan-Based High-Mechanical Double-Network Hydrogels: Construction, Modulation and Applications [J]. Acta Chimica Sinica, 2021, 79(1): 1-9.
[10]	Ma Minghao, Xu Ming, Liu Sijin. Surface Chemical Modifications of Graphene Oxide and Interaction Mechanisms at the Nano-Bio Interface [J]. Acta Chimica Sinica, 2020, 78(9): 877-887.
[11]	Li Haimei, Luo Huajian, Xiao Qi, Yang Liyun, Huang Shan, Liu Yi. Investigations of Interactions and Mechanisms of Chiral Graphene Quantum Dots with DNA [J]. Acta Chimica Sinica, 2020, 78(6): 577-586.
[12]	Jin Fengming, Dong Hongwei, Zhao Yan, Zhuang Shengli, Liao Lingwen, Yan Nan, Gu Wanmiao, Zha Jun, Yuan Jinyun, Li Jin, Deng Haiteng, Gan Zibao, Yang Jinlong, Wu Zhikun. Module Replacement of Gold Nanoparticles by a Pseudo-AGR Process [J]. Acta Chimica Sinica, 2020, 78(5): 407-411.
[13]	Sun Yanhui, Qi Youxiao, Shen You, Jing Cuijie, Chen Xiaoxiao, Wang Xinxing. Preparation of Electrochemical Sensor Based on RGO-Au-ZIF-8 Composite and Its Application in Simultaneous Detection of Lead Ions and Copper Ions [J]. Acta Chimica Sinica, 2020, 78(2): 147-154.
[14]	Zhao Yajing, Xie Liang, Ma Lanchao, He Junhui. Preparation and Application of Polydimethylsiloxane Encapsulated Graphene-based Flexible Infrared Detector [J]. Acta Chimica Sinica, 2020, 78(2): 161-169.
[15]	Li Wei, Ran Tiecheng, Zhang Yu, He Wei, Ma Jifei, Wang Qisheng, Zhang Jichao, Zhu Ying. SiO₂-Mediated High-efficiency Enrichment of 5 nm Gold Nanoparticles and Their Catalytic Activity [J]. Acta Chimica Sinica, 2020, 78(2): 170-176.