Acta Chimica Sinica ›› 1998, Vol. 56 ›› Issue (1): 47-51. Previous Articles     Next Articles

Original Articles

稀土元素对3'-腺嘌呤及3'-鸟嘌呤核苷酸的水解断裂作用

朱兵;李新民;吴亦洁;赵大庆;倪嘉缵   

  1. 中国科学院长春应用化学研究所;中国科学院稀土化学与物理开放实验室
  • 出版日期:1998-01-15 发布日期:1998-01-15

Hydrolytic cleavage of adenosine-3'-monophosphate and Guanosine-3'-monophosphate by Lanthanides

ZHU BING;LI XINMIN;WU YIJIE;ZHAO DAQING;NI JIAZUAN   

  • Online:1998-01-15 Published:1998-01-15

The hydrolytic cleavage of adenosine-3'-monophosphate (3'-AMP) and guanosine-3'-monophosphate (3-GMP) by lanthanides was investigated by NMR, HPLC and the method of measuring the liberated inorganic phosphate. The results show that lanthanides have specificity for hydrolyzing 3'-mononucleotides. 3'-AMP and 3'-GMP were converted to Adenosine(A), phosphate and Guanosine(G), phosphate respectively at pH 9.37℃. The efficiency of cleavage was greater than that of 5'-mononucleotides. The mechanism of hydrolytic of cleavage was discussed in detail.

Key words: HYDROLYSIS, NMR SPECTROMETRY, GUANINE, ADENINE, NUCLEOTIDES

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