Poly-adenine-based DNA Probes and Their Applications in Biosensors★

doi:10.6023/A23040121

Acta Chimica Sinica ›› 2023, Vol. 81 ›› Issue (6): 681-690.DOI: 10.6023/A23040121 Previous Articles

Special Issue: 庆祝《化学学报》创刊90周年合辑

Review

多聚腺嘌呤DNA探针及其生物传感应用^★

李兰英^a, 陶晴^a, 闻艳丽^a, 王乐乐^a, 郭瑞妍^a, 刘刚^a^,^*(), 左小磊^b^,^*()

a 上海市计量测试技术研究院国家市场监督管理总局生物分析计量溯源重点实验室上海 201203
b 上海交通大学医学院分子医学研究院上海 200127

投稿日期:2023-04-08 发布日期:2023-05-31

作者简介:

李兰英, 上海市计量测试技术研究院高级工程师, 主要从事基于polyA探针的生物传感器研究, 主持完成科研项目3项, 主持和参与省部级以上科研课题6项, 在国内外期刊上发表论文20余篇, 包含ACS Appl. Mater. Inter., Biosens. Bioelectron., Anal. Chem.等, 获得发明专利授权10余项.

刘刚, 上海市计量测试技术研究院教授级高工, 研究方向生物标准物质、生物传感方法. 美国国家标准化技术研究院(National Institute of Standard and Technology)访问学者, 获得上海市青年拔尖人才计划资助, 曾获上海市科技进步二等奖, 研发国家有证标准物质30余项, 发表论文50余篇, 其中包含ACS Appl. Mater. Inter., Biosens. Bioelectron., Anal. Chem.等, 获得发明专利授权20余项.

左小磊, 上海交通大学医学院分子医学研究院研究员, 博士生导师, 国家杰出青年科学基金获得者、国家科技部重点专项项目负责人、教育部长江学者奖励计划青年项目获得者、基金委优秀青年科学基金获得者. 曾获得中国分析测试协会一等奖(CAIA奖)、中科院百人计划等. 长期从事框架核酸、生物传感、DNA存储等领域的研究. 在Nature Nanotechnology、Nature Biomedical Engineering、Nature Protocols、Chem. Rev.、JACS, Angew. Chem. Int. Ed.等学术期刊上发表论文150余篇, 论文SCI他引10000余次.

^★庆祝《化学学报》创刊90周年.

基金资助:
国家质量基础设施体系专项(2021YFF0600705); 国家自然科学基金(22074093); 上海市青年科技英才扬帆计划(21YF1459500)

Poly-adenine-based DNA Probes and Their Applications in Biosensors^★

Lanying Li^a, Qing Tao^a, Yanli Wen^a, Lele Wang^a, Ruiyan Guo^a, Gang Liu^a(), Xiaolei Zuo^b()

a Key Laboratory of Bioanalysis and Metrology for State Market Regulation, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China
b Institute of Molecular Medicine, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China

Received:2023-04-08 Published:2023-05-31
Contact: *E-mail: liug@simt.com.cn; zuoxiaolei@sjtu.edu.cn
About author:
^★Dedicated to the 90th anniversary of Acta Chimica Sinica.
Supported by:
National Quality Infrastructure Program of China(2021YFF0600705); National Natural Science Foundation of China(22074093); Shanghai Sailing Program(21YF1459500)

Compared to traditional thiolated DNA probes, poly-adenine-based DNA probes (polyA DNA probes) are free of special chemical modifications, and thus exhibit unique advantages of easy synthesis, low economic cost, and excellent stability. Using their intrinsic polyA fragments, polyA DNA probes are combined onto the gold surface to form a compact and ordered monolayer with both anchoring and recognition capabilities. As a result, polyA DNA probes have attracted numerous research interests in biosensing. In this review, we first presented the mechanism of interaction between polyA DNA probes and the gold surface and then reviewed the applications of the polyA DNA probes in the development of biosensors, including colorimetric biosensors, fluorescence biosensors, surface-enhanced Raman scattering (SERS) biosensors, and electrochemical biosensors. We concluded with a discussion of the opportunities and challenges for polyA DNA probes and expected this review to be informative for the development of biosensors in food safety, environmental monitoring, and biomedicine.

Key words: poly-adenine-based DNA probe, self-assembly, biosensor, biomarker

Cite this article

Lanying Li, Qing Tao, Yanli Wen, Lele Wang, Ruiyan Guo, Gang Liu, Xiaolei Zuo. Poly-adenine-based DNA Probes and Their Applications in Biosensors^★[J]. Acta Chimica Sinica, 2023, 81(6): 681-690.

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Fig. & Tab. 7

Figure 1. (a) Bonding of DNA bases and gold nanoparticles[26]. (b) Idealized schematic of polyA DNA probes on gold[28]

Figure 2. (a) Schematic for spatial control on gold nanoparticles by varying the length of polyA blocks[21]. (b) Enzyme-free colorimetric biosensor based on polyA DNA-conjugated gold nanoparticle-catalyzed color bleaching reaction of methyl orange (MO)[32]. (c) PolyA DNA scaffolds-encoded gold nanoparticle clusters as programmable enzyme equivalents[42]

Figure 3. (a) Schematic for the activity of polyA-based aptamer nanobeacon programmably engineered by tuning the length of polyA[48]. (b) Schematic showing the simultaneous multicolor detection of three microRNAs with polyA50 mediated spherical nucleic acid[30]. (c) Schematic representation of polyA-based aptamer nanobeacon preparation with programable surface density and their kanamycin sensing application[49]. (d) Schematic for polyA-based programmable engineering of gold nanoparticles for highly regulated spherical DNAzymes[50]

Figure 4. (a) Schematic illustration of the polyA-mediated fluorescent spherical nucleic acid (FSNA) probes for c-Myc mRNA detection in living cells[51]. (b) Schematic of the polyA-mediated spherical nucleic acid (pASNA) nanoprobes for microRNAs detection in live cells[54].(c) Schematic illustration for the construction of polyA-spherical nucleic acids with polyA-programed lateral spacing for rescuing the expression of an anti-oncogene[55]

Figure 5. (a) Schematic illustration of the design of the polyA non-fluorescent nanotags (pA-nF-NTs)[60]. (b) Schematic illustration of the polyA-assisted self-assembly strategy for the fabrication of symmetric core (Au)-satellite (Au) nanoassemblies (Au-AuNPs) in colloid[61]. (c) Schematic illustration of DNA conformation modulation with polyA length on multibranched gold nanoparticles (AuNSs) and in situ SERS monitoring of conformation transformation[63]

Figure 6. (a) Schematic illustration of the polyA-based electrochemical DNA biosensor[6]. (b) Schematic illustration of 16S rRNA gene biosensor using a polyA capture probe[68]. (c) Schematic illustration of the triblock polyA probe-based electrochemical DNA biosensor[69]

类型	优势	不足
比色生物传感器	无需巯基己醇等小分子封闭、响应快速、结果直观、适合环境水样监测现场分析	灵敏度不高、易受食品中蛋白和脂肪等干扰物质影响
荧光生物传感器	灵敏度高、特异性好、适合生物医学中细胞成像、细胞内传感	用于信号输出的荧光染料易被光漂白, 影响检测结果的可靠性
表面增强拉曼散射生物传感器	不受光漂白和光谱重叠等外界因素影响、适合生物医学中原位无损分析和成像	成本高、拉曼检测信号重复性和稳定性差, 准确定量分析困难
电化学生物传感器	成本低、易于微型化和集成化、适合食品安全和环境监控现场分析	响应较慢、无法在活体生物中使用

Table 1. Comparison of biosensors based on polyA DNA probes

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多聚腺嘌呤DNA探针及其生物传感应用^★

Poly-adenine-based DNA Probes and Their Applications in Biosensors^★

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多聚腺嘌呤DNA探针及其生物传感应用★

Poly-adenine-based DNA Probes and Their Applications in Biosensors★

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多聚腺嘌呤DNA探针及其生物传感应用^★

Poly-adenine-based DNA Probes and Their Applications in Biosensors^★