Acta Chimica Sinica ›› 2002, Vol. 60 ›› Issue (3): 385-388.     Next Articles

Original Articles

分子烙印亲和色谱与质谱联用实现中草药活性成分分离鉴定一体化

谢建春;朱丽荔;徐筱杰   

  1. 北京大学化学与分子工程学院北京大学养生堂天然药物研究实验室.北京 (100871)
  • 出版日期:2002-03-15 发布日期:2002-03-15

Efficient Separation and Identification of Active Compounds from Herb by Combining Molecular Imprinting Affinity Chromatography with Mass Spectrometry

Xie Jianchun;Zhu Lili;Xu Xiaojie   

  1. College of Chemistry and Molecular Engineering, Beida Yangshengtang Laboratory for Natural Products, Peking University.Beijing(100871)
  • Online:2002-03-15 Published:2002-03-15

To avoid leaking disturbance of the residual template molecules in the application of the molecular imprinted polymer, target analogue (harman) imprinted polymer was prepared with affinity for harmaline and harmine, which exist in Peganum nigellastrum and show antitumor activity. The anti-tumor components, harmaline and harmine, in the crude extract of Peganum nigellastrum were separated and identified simultaneously from the complex matrix by connecting the molecular imprinting affinity column with a positive atmospheric pressure chemical ionization (API) time-of-flight (TOF) mass spectrometry (MS). This result demonstrated the feasibility of efficient separation and identification of active components by combining molecular imprinting affinity liquid chromatography (LC) with mass spectrometry (MS).

Key words: Peganum nigellastrum, ANTITUMOR DRUGS, TEMPLATE AGENT, CHROMATOGRAPHY-MASS SPECTROMETRY, TRADITIONAL CHINESE MEDICINAL HERBS

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