Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (20): 2007-2009. Previous Articles     Next Articles

电位扫描过程中NAD+在银电极上的原位表面增强拉曼光谱

杨海峰, 封婕, 王桂华, 章宗穰   

  1. 上海师范大学生命与环境科学学院化学系, 上海, 200234
  • 收稿日期:2004-02-09 修回日期:2004-04-20 出版日期:2004-10-28 发布日期:2014-02-17
  • 通讯作者: 杨海峰,E-mail:haifengyang@yahoo.com E-mail:haifengyang@yahoo.com
  • 基金资助:
    上海市教委高校青年科学基金(No.98QN77)和上海市教委发展基金(No.03DZ16)资助项目.

In-situ SERS Raman Spectra of NAD+ on Silver Electrode Recorded during a Potential Scanning Procedure

YANG Hai-Feng, FENG Jie, WANG Gui-Hua, ZHANG Zong-Rang   

  1. College of Life and Environment Science, Shanghai Normal University, Shanghai 200234
  • Received:2004-02-09 Revised:2004-04-20 Online:2004-10-28 Published:2014-02-17

In-situ study of NAD+ adsorbed on a chemically roughened silver surface has been conducted using surface enhanced Raman scattering method combined with a confocal technique. The recorded spectra depict that under polarization conditions from 0.4 to 0.2 V, the adenine moiety of NAD+ adopts a perpendicular orientation via the N7 and amino group with respect to the silver surface. In the more negative potential range from 0.1 to -0.2 V, the adenine ring moiety tends to lay on the surface in a flat conjugation. In addition, the adsorption mode of the nicotinamide moiety also shifts with the potential scan. It could be concluded that the adsorption ways of both adenine and nicotinamide moieties are dependent of the applied voltages due to existing a flexible pyrophosphate bridging of them.

Key words: NAD+, confocal Raman, in-situ SERS, silver electrode