The compound, 3',6'-bis(tetramethylethylenedioxyboryl)fluorane, synthesized in this experiment is a kind of H₂O₂ fluorescence-strengthened reagent, which depends on deprotection mechanism and can examine the H₂O₂ concentration sensitively. The structure of the compound was confirmed by the IR and ¹H NMR spectra. The influences of reaction time, solvent and base on the probe synthesis were investigated in detail. The response time of the fluorescent probe was also studied and as a result, the optimal time for the H₂O₂ detection is 3 min. The fluorescence intensity of the probe was gradually increased with the increase of H₂O₂ concentration in the range of 2.3×10^－12～2.0×10^－8 mol/L, the detection limit of H₂O₂ was 2.3×10^－12 mol/L, and the recovery rates in the blood serum were in the range of 93.9%～102.2%.

Key words: 3',6'-bis(tetramethylethylenedioxyboryl)fluorane, fluorescent probe, H₂O₂, deprotection