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Acta Chimica Sinica ›› 2006, Vol. 64 ›› Issue (16): 1700-1704. Previous Articles     Next Articles

Original Articles

耐药相关果糖二磷酸醛缩酶C的生物质谱分析与鉴定

吕磊1,2,3, 刘志强*,1, 李丽1, 刘宁1, 刘淑莹1   

  1. (1中国科学院长春应用化学研究所 长春质谱中心 长春 130022)
    (2延安大学化学系 延安 716000)
    (3中国科学院研究生院 北京 100039)
  • 投稿日期:2005-12-14 修回日期:2006-01-09 发布日期:2006-08-28
  • 通讯作者: 刘志强

Identification and Analysis of Resistance-related Fruc-tose-bisphosphate Aldolase C by Bio-mass Spectrometry

LÜ Lei 1,2,3; LIU Zhi-Qiang*,1; LI Li 1; LIU Ning1; LIU Shu-Ying1   

  1. (1 Changchun Institute of Applied Chemistry, Changchun Center of Mass Spectrometry, Changchun 130022)
    (2 Department of Chemistry, Yan'an Univerisity, Yan'an 716000)
    (3 Graduate School of the Chinese Academy of Sciences, Beijing 100039)
  • Received:2005-12-14 Revised:2006-01-09 Published:2006-08-28
  • Contact: LIU Zhi-Qiang

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Fructose-bisphosphate aldolase C is one of important glycolytic isozymes in the metabolic process. The fructose-bisphosphate aldolase C which highly expressed in the human ovarian adenocarcinoma cell has been separated by two-dimensional gel electrophoresis and identified by matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF MS). The results indicate that MALDI-TOF/TOF MS in combination with the high resolution two dimensional gel electrophoresis is a simple and fast method for identification of unknown proteins.

Key words: aldolase C, matrix-assisted laser desorption ionization tandem time of flight mass spectrometry, two dimensional gel electrophoresis