Study on the On-line Evaluation Method for Antioxidants by Capillary Electrophoresis with Indirect Chemiluminescence Detection

Acta Chimica Sinica ›› 2006, Vol. 64 ›› Issue (23): 2344-2350. Previous Articles Next Articles

Original Articles

抗氧化剂的毛细管电泳-间接化学发光联用在线评价方法研究

郑国灿¹, 陈华¹,2, 陈志涛¹, 甘婷婷², 冯波¹, 夏之宁*^,1,2

(¹重庆大学化学化工学院重庆 400044)
(²重庆大学生物工程学院重庆 400044)

投稿日期:2006-01-26 修回日期:2006-06-20 发布日期:2006-12-14
通讯作者: 夏之宁

Study on the On-line Evaluation Method for Antioxidants by Capillary Electrophoresis with Indirect Chemiluminescence Detection

ZHENG Guo-Can¹; CHEN Hua¹,2; CHEN Zhi-Tao¹; GAN Ting-Ting²; FENG Bo¹; XIA Zhi-Ning*^,1,2

(¹ College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044)
(²College of Bioengineering, Chongqing University, Chongqing 400044)

Received:2006-01-26 Revised:2006-06-20 Published:2006-12-14
Contact: XIA Zhi-Ning

Abstract

A novel method of on-line evaluation of antioxidants by capillary electrophoresis (CE) with indirect chemiluminescence (CL) detection was reported. It is based on the CL of luminol suppressed for lessened hydroxy radical which is scavenged by antioxidants in base condition. Optimal chemiluminescence conditions were obtained, and the antioxidant activities of thiourea and ephedrine were evaluated. The IC₅₀ values of thiourea and ephedrine were obtained by fitting curves, and the order of antioxidant activity is ephedrine＞thiourea. The result was consistent with that of fluorospectrophotometry. The proposed method was preliminarily applied to evaluating the antioxidant activity of components of Sophora japonica L.

Key words: capillary electrophoresis, chemiluminescence, antioxidant, evaluation, on-line

Cite this article

ZHENG Guo-Can; CHEN Hua^1,2; CHEN Zhi-Tao; GAN Ting-Ting²; FENG Bo; XIA Zhi-Ning*^,1,2. Study on the On-line Evaluation Method for Antioxidants by Capillary Electrophoresis with Indirect Chemiluminescence Detection[J]. Acta Chimica Sinica, 2006, 64(23): 2344-2350.

Export EndNote|Reference Manager|ProCite|BibTeX|RefWorks

[1]	Jinyuan Zhao, Qian Zhang, Jian Wang, Qi Zhang, Heng Li, Yaping Du. Advances in the Scavenging Materials for Reactive Oxygen Species [J]. Acta Chimica Sinica, 2022, 80(4): 570-580.
[2]	Liu Jiao, Sun Hailong, Yin Lu, Yuan Yaxian, Xu Minmin, Yao Jianlin. On-line Monitoring on the Micro-synthesis of α-Phenylethanol by Microfluidic Chip Combined with Surface Enhanced Raman Spectroscopy [J]. Acta Chim. Sinica, 2019, 77(3): 257-262.
[3]	Zhang Zhaoxiang, Liu Yujie, Wang Qi, Wang Jingjing. Capillary Electrophoresis and Quantum Dot Electrochemiluminescence by Micellar Reversed Sweeping [J]. Acta Chim. Sinica, 2019, 77(2): 179-183.
[4]	Zhang Zhaoxiang, Luan Wenxiu, Zhang Chaoying, Liu Yujie. Capillary Electrophoresis Immunoassay by Gold Nanoparticles Assisted Signal Generation and Sequential Stacking [J]. Acta Chim. Sinica, 2017, 75(4): 403-407.
[5]	Zhu Benzhan, Shao Bo, Mao Li, Gao Huiying. Intrinsic Chemiluminescence Production during Environmentally-friendly Advanced Oxidation of Halogenated Aromatics and Its Applications [J]. Acta Chim. Sinica, 2016, 74(7): 557-564.
[6]	Li Yuan, Liu Wenna, Zheng Xingwang. Investigation into Electrogenerated Chemiluminescence Behavior of Tris(bipyridine)ruthenium(II)/silica Nanoparticles Electrocatalyzed by Cu(tris(hydroxymethyl)aminomethane)₄²⁺ Complex [J]. Acta Chim. Sinica, 2015, 73(7): 749-754.
[7]	Wang Dabin, Zhao Lixia, Guo Lianghong, Zhang Hui, Wan Bin, Yang Yu. Online Quantification of O₂^·- and H₂O₂ and Their Formation Kinetics in Ultraviolet (UV)-Irradiated Nano-TiO₂ Suspensions by Continuous Flow Chemiluminescence [J]. Acta Chim. Sinica, 2015, 73(5): 388-394.
[8]	Zhang Lihong, Guo Zhihui. Study on the Inhibition Effect of Carbon Quantum Dots on Luminol Electrochemiluminescence System and Its Application in DNA Analysis [J]. Acta Chimica Sinica, 2013, 71(04): 644-648.
[9]	Wei Wei, Zhang Quan, Zheng Xingwang. Synthesis of Chitosan/Fe₃O₄/SiO₂ Nanocomposites and Investigation into Their Catalysis Properties [J]. Acta Chimica Sinica, 2013, 71(03): 387-391.
[10]	Liu Chenchen, Li Zhenqing, Meng Fanming, Ni Yi, Dou Xiaoming, Yamaguchi Yoshinori. Capillary Electrophoresis of DNA in Hydroxyethylcellulose [J]. Acta Chimica Sinica, 2013, 71(02): 265-270.
[11]	Tong Bihai, Mei Qunbo, Li Zhiwen, Dong Yongping, Zhang Qianfeng. Investigation on the Electrochemiluminescence Properties of a Series of Cyclometalated Iridium(III) Complexes Based on 2-Phenylquinoline Derivatives [J]. Acta Chimica Sinica, 2012, 70(23): 2451-2456.
[12]	Zhang Zhaoxiang, Zhang Fei, Liu Ying. Electrochemical Immunoassay by Capillary Electrophoresis for E. coli Enhanced by Field-ampli?ed Sample Injection and Gold Nanoparticles [J]. Acta Chimica Sinica, 2012, 70(21): 2251-2256.
[13]	Chen Jin, Li Zhenqing, Ni Yi, Liu Chenchen, Dou Xiaoming, Yamaguchi Yoshinori. Effect of Electric Field Modes on the Separation Performance of DNA in Capillary Electrophoresis [J]. Acta Chimica Sinica, 2012, 70(19): 2073-2078.
[14]	Yang Fan, Wang Lingli, Guo Zhihui. Study on the Influence of Carbon Quantum Dots on Electrogenerated Chemiluminescence Energy Transfer Sensing System of DNA [J]. Acta Chimica Sinica, 2012, 70(11): 1283-1288.
[15]	Du Jianxiu, Wang Hong. Chemiluminescence of Nano-Colloidal MnO₂ with Formaldehyde and Its Analytical Application [J]. Acta Chimica Sinica, 2012, 0(05): 537-543 .

抗氧化剂的毛细管电泳-间接化学发光联用在线评价方法研究

Study on the On-line Evaluation Method for Antioxidants by Capillary Electrophoresis with Indirect Chemiluminescence Detection

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments