Capillary Electrophoresis and Quantum Dot Electrochemiluminescence by Micellar Reversed Sweeping

doi:10.6023/A18090382

Acta Chim. Sinica ›› 2019, Vol. 77 ›› Issue (2): 179-183.DOI: 10.6023/A18090382 Previous Articles Next Articles

Article

基于胶束反向扫集的毛细管电泳量子点电化学发光研究

张召香^a, 刘玉洁^a, 王琪^a, 王静静^b

a 光电传感与生命分析教育部重点实验室山东省生化分析重点实验室 “十三五”高校生命分析化学重点实验室青岛科技大学化学与分子工程学院青岛 266042;
b 山东出入境检验检疫局检验检疫技术中心青岛 266500

投稿日期:2018-09-11 发布日期:2018-11-26
通讯作者: 张召香,E-mail:qustzhzhx@126.com E-mail:qustzhzhx@126.com
基金资助:
项目受国家自然科学基金（No.21105051）资助.

Capillary Electrophoresis and Quantum Dot Electrochemiluminescence by Micellar Reversed Sweeping

Zhang Zhaoxiang^a, Liu Yujie^a, Wang Qi^a, Wang Jingjing^b

a Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE;Shandong Key Laboratory of Biochemical Analysis;Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong;College of Chemistry and Molecular Engineering. Qingdao University of Science and Technology, Qingdao 266042, China;
b Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266500, China

Received:2018-09-11 Published:2018-11-26
Contact: 10.6023/A18090382 E-mail:qustzhzhx@126.com
Supported by:
Project supported by the National Natural Science Foundation of China (No. 21105051).

1. Capillary Electrophoresis and Quantum Dot Electrochemiluminescence by Micellar Reversed Sweeping.PDF(261KB)

Abstract

The strategy for electrochemiluminescence (ECL) sensor based on the CdSe quantum dots (QDs) to detect amines is proposed. We investigated the QDs ECL toward different amines, and found that amines could lead to the enhancement of ECL intensity. A novel amines detection platform based on micellar reversed sweeping, capillary electrophoresis (CE) separation, and quantum dots electrochemiluminescence detection was proposed for simultaneous detection of ractopamine and clenbuterol in meat samples. Firstly, the capillary was filled with running buffer containing SDS micelles. The electrophoretic velocity of SDS micelle was reverse to that of electroosmotic velocity. By controlling electroosmotic flow, the SDS-sample conjugates were at an immobile state in capillary. This immobile state was maintained for an extended period of time so that essentially unlimited volume of sample solution could be injected into the capillary. Then the sample was electrokinetically introduced into the separation capillary at 20 kV for 50 min. The negative charged SDS micelles in the buffer attracted the sample ions at the boundary of sample and buffer solution. The micellar reversed sweeping process allows introducing large amount of analytes into capillary to accumulate at the capillary inlet. After CE separation, the preconcentrated analytes sequentially enter into detection cell and lead to the enhancement of ECL intensity of QDs. The micellar reversed sweeping allows a large number of analytes trapped by SDS micelles, which could significantly amplify the QD's ECL signal for 6000-fold. The proposed method by micellar reversed sweeping and CE separation with QDs ECL detection realized the simultaneous and sensitive determination of ractopamine and clenbuterol in meat samples. The linear range were (0.8~2960) and (3.0~5520) μg/L and the limit of detection (LOD) were 96.8 and 192.5 ng/L for ractopamine and clenbuterol, respectively. CE based QDs ECL that combines the high separation efficiency of CE and the high sensitivity of QDs ECL has been proven to be a promising technique for amines compound assays.

Key words: micellar reversed sweeping, capillary electrophoresis, quantum dots, electrochemiluminescence, amines

Cite this article

Zhang Zhaoxiang, Liu Yujie, Wang Qi, Wang Jingjing. Capillary Electrophoresis and Quantum Dot Electrochemiluminescence by Micellar Reversed Sweeping[J]. Acta Chim. Sinica, 2019, 77(2): 179-183.

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基于胶束反向扫集的毛细管电泳量子点电化学发光研究

Capillary Electrophoresis and Quantum Dot Electrochemiluminescence by Micellar Reversed Sweeping

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