To prepare the molecularly imprinted membrane with capacity of specifically absorbing the template chloramphenicol succinate, photo-polymerization method was applied to prepare the molecularly imprinted polymers by using chloramphenicol succinate as template, MA as functional monomer, EGDMA as cross-linking agent, AIBN as initiator and tetrahydrofuran as solvent. Then through phase inversion, cellulose acetate membrane with imprinted sites was made using chloramphenicol succinate imprinted polymers. The absorptive characteristics of this membrane was identified by absorbing experiment. Compared with non-imprinted membrane, imprinted membrane has a good capacity of specifically absorbing the template molecules. The concentration of chloramphenicol succinate solvent changed significantly after being interacted with imprinted membrane. Most of this recognizing reactions were completed in the first 2 h and declined as the reaction time prolonged. The higher the concentration of template in the scope of 0.2～0.0125 mg/mL, the more significant the capacity of the imprinted polymers. The imprinted membrane made in this research had high efficiency towards the separation of template molecule and was proposed to use in the future study for the creation of chloramphenicol detection sensor.

Key words: chloramphenicol, molecular imprinted membrane, absorptive characteristics