Fluorescent Aptamer-functionalized Graphene Oxide Biosensor for Rapid Detection of Chloramphenicol

doi:10.6023/A18100433

Acta Chim. Sinica ›› 2019, Vol. 77 ›› Issue (3): 253-256.DOI: 10.6023/A18100433 Previous Articles Next Articles

Special Issue: 分子探针、纳米生物学与生命分析化学

Communication

荧光核酸适配体功能化氧化石墨烯生物传感器用于快速检测氯霉素

卢静荷, 谭淑珍, 朱雨清, 李伟, 陈天啸, 王瑶, 刘陈

长沙理工大学化学与生物工程学院长沙 410114

投稿日期:2018-10-17 发布日期:2019-01-17
通讯作者: 谭淑珍 E-mail:tsz519@163.com
基金资助:
项目受湖南省教育厅科研基金（No.17C0033）资助.

Fluorescent Aptamer-functionalized Graphene Oxide Biosensor for Rapid Detection of Chloramphenicol

Lu Jinghe, Tan Shuzhen, Zhu Yuqing, Li Wei, Chen Tianxiao, Wang Yao, Liu Chen

School of Chemical and Biological Engineering, Changsha University of Science and Technology, Changsha 410114

Received:2018-10-17 Published:2019-01-17
Supported by:
Project supported by the Scientific Research Fund of Hunan Provincial Education Department (No. 17C0033).

1. Fluorescent Aptamer-functionalized Graphene Oxide Biosensor for Rapid Detection of Chloramphenicol.PDF(701KB)

Abstract

A label free and rapid fluorescent method for quantitative detection of chloramphenicol (CAP) based on graphene oxide (GO) fluorescence functional G-quadruplex probe (FGP) was developed. The FGP consisted of a choramphenicol aptamer and a G-rich sequence. The aptamer was used to bind CAP and the G-quadruplex formed by G-rich sequence was employed as a signal reporter after binding to Thioflavin T (ThT). In the absence of CAP, the FGP was absorbed onto the surface of GO through π-π stacking interactions, which restrained the G-rich sequence to form a G-quadruplex structure. Thus, the fluorescent intensity of background was low. In the addition of the CAP, the aptamer part of FGP could recognize and bind CAP to form a target-FGP complex, which led to the desorption of the complex from GO. Therefore, the free G-rich sequence could form G-quadruplex structure and bind to ThT, resulting a increase in the fluorescence intensity of the solution. We observed that the fluorescence increasement of the sensing platform had a linear relationship with the concentrations of CAP in the range of 2~20 nmol/L, and the limit of detection was 1.45 nmol/L. Besides, this detection system was applied for detecting CAP in the spiked milk, the recovery rate was between 93.2%~103.3%. These results indicated that this developed method can be used to efficiently recognize CAP in real samples.

Key words: aptamer, fluorescence, chloramphenicol, G-quadruplex, graphene oxide

Cite this article

Lu Jinghe, Tan Shuzhen, Zhu Yuqing, Li Wei, Chen Tianxiao, Wang Yao, Liu Chen. Fluorescent Aptamer-functionalized Graphene Oxide Biosensor for Rapid Detection of Chloramphenicol[J]. Acta Chim. Sinica, 2019, 77(3): 253-256.

Export EndNote|Reference Manager|ProCite|BibTeX|RefWorks

share this article

References

[1] Wang, L.; Zhang, Y.; Gao, X.; Duan, Z.; Wang, S. J. Agric. Food Chem. 2010, 58, 3265.
[2] Wang, H.; Zhou, X.; Liu, Y.; Yang, H.; Guo, Q. J. Agric. Food Chem. 2011, 59, 3532.
[3] Webb, S.; Ternes, T.; Gibert, M.; Olejniczak, K. Toxicol. Lett. 2003, 142, 157.
[4] Duan, Y.; Wang, L.; Gao, Z. Talanta 2017, 165, 671.
[5] Tan, Z.; Xu, H.; Li, G. Spectrochim. Acta A 2015, 149, 615.
[6] Pfenning, A. P.; Roybal, J. E.; Rupp, H. S.; Turnipseed, S. B.; Gonzales, S. A.; Hurlbut, J. A. J. AOAC Int. 2000, 83, 26.
[7] Wang, L.; Hai, Y.; Zhang, C. Anal. Chim. Acta 2008, 619, 54.
[8] Kawalski, D.; Pobo?y, E.; Trojanowicz, M. J. Autom. Methods Manag. Chem. 2011, 8565, 143416.
[9] Ramos, M.; Muñoz, P.; Aranda, A.; Rodriquez, I.; Diaz, R.; Blanca, J. J. Chromatogr. B 2003, 791, 31.
[10] Tian, H. Chemosphere 2011, 83, 349.
[11] Kolosova, A. Y.; Samsonova, J. V.; Egorova, M. Food Agr. Immunol. 2000, 12, 115.
[12] Byzova, N. A.; Zvereva, E. A.; Zherdev, A. V.; Eremin, S. A.; Dzantiev, B. B. Talanta 2010, 81, 843.
[13] He, J. L.; Zhu, S. L.; Wu, P.; Li, P. P.; Li, T.; Cao, Z. Biosens. Bioelectron. 2014, 60, 112.
[14] Chen, M.; Gan, N.; Zhang, H. Microchim. Acta 2016, 183, 1099.
[15] Gao, H.; Pan, D.; Gan, N. Microchim. Acta 2015, 182, 2551.
[16] Miao, Y. B.; Ren, H. X.; Gan, N. Anal. Chim. Acta 2016, 929, 49.
[17] Miao, Y. B.; Gan, N.; Li, T. H.; Zhang, H. R.; Cao, Y. T.; Jiang, Q. L. Sensor. Actuat. B-Chem. 2015, 220, 679.
[18] Sharma, R.; Ragavan, K. V.; Raghavarao, K. S. M. S. Biotechnology and Biochemical Engineering, Springer, Sin-gapore, 2016.
[19] Zhao, K.; Hao, Y.; Zhu, M.; Cheng, G. Acta Chim. Sinica 2018, 76, 168(in Chinese). (赵克丽, 郝莹, 朱墨, 程国胜, 化学学报, 2018, 76, 168.)
[20] Sun, H.; He, Q.; Yin, S.; Xu, K. Chin. J. Chem. 2017, 35, 1627.
[21] Yang, X.; Cai, H.; Bao, M.; Yu, J.; Lu, J.; Li, Y. Chin. J. Chem. 2017, 35, 1549.
[22] Chen, X. J.; Wang, Y. Z.; Zhang, Y. Y.; Chen, Z. H.; Liu, Y.; Li, Z. L.; Li, J. H. Anal. Chem. 2014, 86, 4278.
[23] Liu, X.; Wang, F.; Aizen, R. J. Am. Chem. Soc. 2013, 135, 11832.
[24] Yi, M.; Yang, S.; Peng, Z. Y.; Liu, C. H.; Li, J. S.; Zhong, W. W.; Yang, R. H.; Tan, W. H. Anal. Chem. 2014, 86, 3548.
[25] Zhang, P.; Gong, J. L.; Zeng, G. M.; Deng, C. H.; Yang, H. C.; Liu, H. Y.; Huan, S. Y. Chem. Eng. J. 2017, 322, 657.
[26] Luu, K. N.; Phan, A. T.; Kuryavyi, V. J. Am. Chem. Soc. 2006, 128, 9963.
[27] Guo, Y.; Sun, Y.; Shen, X.; Chen, X.; Yao, Y. R.; Xie, Y. F.; Hu, J. M.; Pei, R. J. Anal. Methods 2015, 7, 9615
[28] Vummidi, B. R.; Alzeer, J.; Luedtke, N. W. ChemBioChem 2013, 14, 540.
[29] Zhu, L. N.; Wu, B.; Kong, D. M. Nucleic Acids Res. 2013, 41, 4324.
[30] Huang, X. X.; Zhu, L. N.; Wu, B. Nucleic Acids Res. 2014, 42, 8719.
[31] Zhang, Q.; Liu, Y. C.; Kong, D. M. Chem. Eur. J. 2015, 21, 13253.
[32] Jiang, H. X.; Liang, Z. Z.; Ma, Y. H. Anal. Chim. Acta 2016, 943, 114.
[33] Mohanty, J.; Barooah, N.; Dhamodharan, V. J. Am. Chem. Soc. 2012, 135, 367.
[34] Zhang, Q. J.; Peng, T.; Chen, D. D. J. Aoac. Int. 2013, 96, 897.
[35] Miao, Y. B.; Ren, H. X.; Gan, N.; Cao, Y. T.; Li, T. H.; Chen, Y. J. Biosens. Bioelectron. 2016, 81, 454.
[36] Fei, X.; Zhao, F.; Li, J. Anal. Chim. Acta 2007, 596, 79.
[37] Gao, H.; Gan, N.; Pan, D. Anal. Methods 2015, 7, 6528.

荧光核酸适配体功能化氧化石墨烯生物传感器用于快速检测氯霉素

Fluorescent Aptamer-functionalized Graphene Oxide Biosensor for Rapid Detection of Chloramphenicol

PDF

Supporting Info.

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Fengjie Ge, Kaizhi Zhang, Qingpeng Cao, Hui Xu, Tao Zhou, Wenhao Zhang, Xinxin Ban, Xiaobo Zhang, Na Li, Peng Zhu. Design, Synthesis and Electroluminescence Performance of Flexible Fluorenyl Block Delayed Fluorescence Dimers [J]. Acta Chimica Sinica, 2023, 81(9): 1157-1166.
[2]	Yinuo Wang, Shiyang Shao, Lixiang Wang. Recent Advances in Multiple Resonance Organic/Polymer Fluorescent Materials with Narrowband Emission^★ [J]. Acta Chimica Sinica, 2023, 81(9): 1202-1214.
[3]	Feida Che, Xiaoming Zhao, Xin Zhang, Qi Ding, Xin Wang, Ping Li, Bo Tang. Fluorescence Imaging of Active Molecules Associated with Depression^★ [J]. Acta Chimica Sinica, 2023, 81(9): 1255-1264.
[4]	Xiao Wang, Xingwen Wang, Lehui Xiao. Nanocatalytic Mechanisms Investigated by Single Molecule Fluorescence Imaging at the Single-Particle Level [J]. Acta Chimica Sinica, 2023, 81(8): 1002-1014.
[5]	Hongyue Wu, Rui Guo, Hanwen Chi, Yonghe Tang, Sirui Song, Enxiang Ge, Weiying Lin. Viscosity Fluorescent Probes Based on Quinoline Group and Its Applications [J]. Acta Chimica Sinica, 2023, 81(8): 905-911.
[6]	Wenshan Zheng, Guanbin Gao, Hao Deng, Taolei Sun. Room Temperature Synthesis and Near-infrared Fluorescence Performance Optimization of Ag₂Se@Ag₂S Core-shell Quantum Dots [J]. Acta Chimica Sinica, 2023, 81(7): 763-770.
[7]	Zhenyu Liu, Junfeng Rao, Shoujia Zhu, Bingyang Wang, Fan Yu, Quanyou Feng, Linghai Xie. Research Progress of Solution-Processed Self-Host Thermally Activated Delayed Fluorescence Materials [J]. Acta Chimica Sinica, 2023, 81(7): 820-835.
[8]	Yinfeng Wang, Meng Li, Chuanfeng Chen. Chiral Triptycene-Based Red Thermally Activated Delayed Fluorescence Polymers and Their Organic Light-Emitting Diodes^★ [J]. Acta Chimica Sinica, 2023, 81(6): 588-594.
[9]	Xin Lv, Yi Wu, Boran Zhang, Wei Guo. Design, Synthesis and Photodynamic Therapy of a H₂O₂-Activatable Near Infrared Borondipyrromethene （BODIPY） Photosensitizer [J]. Acta Chimica Sinica, 2023, 81(4): 359-370.
[10]	Shaoqin Zhang, Meiqing Li, Zhongjun Zhou, Zexing Qu. Theoretical Study on the Multiple Resonance Thermally Activated Delayed Fluorescence Process [J]. Acta Chimica Sinica, 2023, 81(2): 124-130.
[11]	Yanqin Huang, Lijun Li, Shupei Yang, Rui Zhang, Xingfen Liu, Quli Fan, Wei Huang. HA-AuNPs/FDF for Highly Sensitive Detection of Hyaluronidase, Tumor-targeting Fluorescence Cell Imaging and Phototherapy [J]. Acta Chimica Sinica, 2023, 81(12): 1687-1694.
[12]	Li Sun, Yajing Wang, Tao Li, Yingshu Guo, Shusheng Zhang. Au Nanocages Probes for Mitochondrial Imaging and Photothermal Damage Cells^★ [J]. Acta Chimica Sinica, 2023, 81(10): 1301-1310.
[13]	Wen Liu, Yujie Wang, Huiqin Yang, Chengjie Li, Na Wu, Yang Yan. The Preparation of Carbon Nanotubes/Reduced Graphene Oxide Current Collector by Non-covalent Induction of Ionic Liquid for Sodium Metal Anode [J]. Acta Chimica Sinica, 2023, 81(10): 1379-1386.
[14]	Sirui Song, Yonghe Tang, Liangguang Sun, Rui Guo, Guanfan Jiang, Weiying Lin. Development of a Novel Fluorescent Probe Based on Coumarin Fluorophore for Polarity Detection and Its Imaging Applications [J]. Acta Chimica Sinica, 2022, 80(9): 1217-1222.
[15]	Jie Zhao, Zhiwen Wang, Huaqing Li, Qi Ai, Peiqing Cai, Junjie Si, Xin Yao, Xiaoguang Hu, Zugang Liu. Synthesis and Properties of Visible-light-driven Fluorescence Turn-on Diarylethenes Based on Benzo[b]naphtho[1,2-d]thiophene [J]. Acta Chimica Sinica, 2022, 80(9): 1223-1230.