Acta Chimica Sinica ›› 2009, Vol. 67 ›› Issue (23): 2727-2731. Previous Articles     Next Articles

Full Papers

一种新型分子印迹聚合物基的化学发光阵列传感器

章竹君, 常平平   

  1. (陕西师范大学化学与材料科学学院 西安 710062)
  • 收稿日期:2009-03-27 修回日期:2009-06-21 出版日期:2009-12-14 发布日期:2009-07-24
  • 通讯作者: 章竹君 E-mail:zzj18@hotmail.com
  • 基金资助:

    国家自然科学基金

A Novel Chemiluminescence Array Sensor Based on Molecularly Imprinted Polymer

Chang, Pingping,Zhang, Zhujun   

  1. (School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062)
  • Received:2009-03-27 Revised:2009-06-21 Online:2009-12-14 Published:2009-07-24

A new molecularly imprinted polymer (MIP)-chemiluminescence (CL) detection approach towards recognition of dansyl-glycine (Gly) is presented. The polymer microspheres were synthesized using precipitation polymerization with glycine as template and immobilized in microtiter plates (96 wells) using poly(vinyl alcohol) (PVA) as glue. The analyte (dansyl-glycine) was selectively adsorbed on the MIP microspheres. After washing, the bound fraction was quantified based on a dansyl-glycine-bis- (2,4,6-trichlorophenyl) oxalate (TCPO)-H2O2 chemiluminescence reaction enhanced by imidazole. The chemiluminescence intensity has a linear relationship with the concentration of glycine in the range of 0.2~60 μmol/L, with the limit of detection (LOD) being 0.07 μmol/L (3σ). The relative standard deviation (RSD) for 11 parallel measurements of glycine (1 μmol/L) was 3.3%. The cavity of MIP synthesized with glycine as template is smaller, which can avoid non-specific adsorption and lead to enhancement of specificity, response speed and sensitivity when recognizing dansyl-glycine.

Key words: molecularly imprinted polymer, chemiluminescence, array sensor, glycine