Effect of pH, Quercetin and Zinc Ion on the Structure of β-Casein by Circular Dichroism Method

Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (14): 1449-1453. Previous Articles Next Articles

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圆二色光谱法研究酸度、槲皮素、锌离子对β-酪蛋白二级结构的影响

吕鉴泉*^{,1，王雪梅¹，吕桂英²，孙婷荃¹，唐金燕¹，徐明波¹}

(¹湖北师范学院污染物分析与资源化技术湖北省重点实验室黄石 435002)
(²黄石理工学院化学与材料工程学院黄石 435003)

投稿日期:2009-08-05 修回日期:2010-01-30 发布日期:2010-03-29
通讯作者: 吕鉴泉 E-mail:jianquanlu@eyou.com
基金资助:
教育部重点项目(206096);湖北省教育厅创新团队项目和重点项目(D200922005)

Effect of pH, Quercetin and Zinc Ion on the Structure of β-Casein by Circular Dichroism Method

Lü Jianquan*^{,1 Wang Xuemei¹ Lü Guiying² Sun Tingquan¹ Tang Jinyan¹ Xu Mingbo¹}

(¹ Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Hubei Normal University, Huangshi 435002)
(² School of Chemical and Material Engineering, Huangshi Institute of Technology, Huangshi 435003)

Received:2009-08-05 Revised:2010-01-30 Published:2010-03-29
Contact: LU JIAN-QUAN E-mail:jianquanlu@eyou.com

Abstract

The effect of pH, quercetin (Qct) and Zn(II) on the structure of β-casein (β-CN) in solution were investigated using circular dichroism spectroscopy. The results indicated that the secondary structures of β-CN in the solution were induced by the pH, quercetin and Zn(II)-Qct complexes. In pH 7.6 PBS buffer, β-CN contain 32.6% α-helix, 53.5% β-sheet and 13.9% γ-turn and random coil, the addition of Qct cause increase the content of α-helix and decrease the content of β-sheet, while Zn(II) and Zn(II)-Qct complexes lead to formation of γ-turn and random coil with the decease of α-helix.

Key words: secondary structure, β-casein, circular dichroism, quercetin, Zn(II)

CLC Number:

O657.39

Cite this article

LV Jian-Quan , WANG Xue-Mei, 吕Gui-Ying , SUN Ting-Quan, TANG Jin-Yan, XU Ming-Bo. Effect of pH, Quercetin and Zinc Ion on the Structure of β-Casein by Circular Dichroism Method[J]. Acta Chimica Sinica, 2010, 68(14): 1449-1453.

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圆二色光谱法研究酸度、槲皮素、锌离子对β-酪蛋白二级结构的影响

Effect of pH, Quercetin and Zinc Ion on the Structure of β-Casein by Circular Dichroism Method

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