Cleavage Activity of PVP Amphiphilic Block Copolymer for Supercoiled DNA

Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (16): 1941-1945. Previous Articles Next Articles

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PVP两亲性嵌段共聚物对超螺旋DNA的裂解活性

张立芬*^,1,2，梁勇³，孟令芝²

(¹兰州大学化学化工学院兰州 730000)
(²武汉大学化学与分子科学学院武汉 430072)
(³江汉大学医学与生命科学学院武汉 430056)

投稿日期:2010-05-30 修回日期:2011-03-16 发布日期:2011-04-15
通讯作者: 张立芬 E-mail:lfzhang@lzu.edu.cn
基金资助:
国家自然科学基金项目;教育部博士点基金

Cleavage Activity of PVP Amphiphilic Block Copolymer for Supercoiled DNA

Zhang Lifen*^,1,2 Liang Yong³ Meng Lingzhi²

(¹College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000)
(²College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072)
(³School of Medicine and Life Science, Jianghan University, Wuhan 430056)

Received:2010-05-30 Revised:2011-03-16 Published:2011-04-15
Contact: Lifen Zhang E-mail:lfzhang@lzu.edu.cn

Amphiphilic block copolymers poly(N-vinylpyrrolidone)-block-poly(N-t-Boc-tryptophanamido- N-methacryl thiourea) (PVP-b-PTrpAMT-I, II, III) were designed and synthesized with well defined structures by atom transfer radical polymerization (ATRP) using PVP-Cl as macroinitiator. The chemical structures of the block copolymers were characterized by ¹H NMR and GPC-MALLS. Agarose gel electrophoresis study demonstrated that PVP-b-PTrpAMT can induce and cleavage supercoiled DNA into nicked DNA. The nicked DNA can avoid the degradation of DNA in the cell efficiently and have a potential ability to enhance the gene transfection. The cleavage activity of these block copolymers was obviously dependent on the different composition of the copolymer, quality ratio of the copolymer with plasmid DNA and the incubating time together with plasmid DNA.

Key words: poly(N-vinylpyrrolidone), block copolymer, DNA, cleavage activity

Cite this article

ZHANG Li-Fen, LIANG Yong, MENG Ling-Zhi. Cleavage Activity of PVP Amphiphilic Block Copolymer for Supercoiled DNA[J]. Acta Chimica Sinica, 2011, 69(16): 1941-1945.

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PVP两亲性嵌段共聚物对超螺旋DNA的裂解活性

Cleavage Activity of PVP Amphiphilic Block Copolymer for Supercoiled DNA

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