An Approach for Irreversible Bonding of PDMS-PS Hybrid Microfluidic Chips at Room Temperature

doi:10.6023/A13060678

Acta Chimica Sinica ›› 2013, Vol. 71 ›› Issue (11): 1535-1539.DOI: 10.6023/A13060678 Previous Articles Next Articles

Article

聚二甲基硅氧烷-聚苯乙烯复合微流控芯片室温不可逆封合法的研究

胡贤巧, 何巧红, 白泽清, 苏法铭, 陈恒武

浙江大学化学系微分析系统研究所杭州 310058

投稿日期:2013-06-28 发布日期:2013-08-12
通讯作者: 何巧红, 陈恒武 E-mail:heqh@zju.edu.cn;hwchen@zju.edu.cn
基金资助:
项目受国家重点基础研究发展计划(973, No. 2007CB714502)和国家自然科学基金(No. 20890020)资助.

An Approach for Irreversible Bonding of PDMS-PS Hybrid Microfluidic Chips at Room Temperature

Hu Xianqiao, He Qiaohong, Bai Zeqing, Su Faming, Chen Hengwu

Department of Chemistry, Institute of Microanalytical Systems, Zhejiang University, Zijin'gang Campus, Hangzhou 310058

Received:2013-06-28 Published:2013-08-12
Supported by:
Project supported by the National Basic Research Program of China (973 Program, No. 2007CB714502) and the National Natural Science Foundation of China (No. 20890020).

In some circumstances, hybrid polymer microfluidic chips composed of both elastic, gas-permeable polydimethylsiloxane (PDMS) and rigid plastics are needed. However, it is quite difficult to bond PDMS irreversibly to plastics such as polystyrene (PS). In this article, a facile method for irreversible bonding of PDMS to PS was proposed based on UV/O₃-assisted surface modification in combination of surface silanization. Firstly, a PS sheet was exposed to UV/O₃ to produce oxygen-containing polar moieties, such as hydroxyl and carboxylic acid, on its surface. Secondly, the UV/O₃ treated PS sheet was silanized with (3-aminopropyl)triethoxysilane (APTES) via the reaction between the oxygen-containing polar moieties on the PS surface and the molecules of APTES. Thirdly, the silanized-PS sheet and the PDMS substrate with micro channel network were treated with UV/O₃ again to generate silanol moieties on both surfaces. Finally, the UV/O₃treated PDMS was immediately brought intimate contact with the UV/O₃ treatedsilanized-PS, and irreversible bonding of PDMS with PS occurred after putting the PDMS-PS complex at room temperature for 1 h through the condensation reaction between silanol moieties. Contact angle measurement, X-ray photoelectron spectroscopy, total reflection Fourier transformation infrared spectrometer were applied to characterize the surface chemistry of the PS during the UV/O₃ treatment and silanization. The hybrid PDMS-PS microfludic chips prepared with the established method can bear a gas pressure higher than 0.5 MPa and a water stream at a flow rate higher than 170 μL/min (the test channels were 2.5 cm in the length, 50 μm in the width, 200 μm in the depth). A hybrid PDMS-PS microfluidic chip composed of gas-permeable PDMS substrate with channel network and excellently biocompatible PS cover sheet was fabricated for cell culture. The experimental results showed that HeLa cells cultured in the hybrid PDMS-PS microchip grew much better than those cultured in the full PS microchip, and slightly better than those in the full PDMS microchip.

Key words: hybrid microfluidic chips, polydimethylsiloxane, polystyrene, irreversible bonding, cell culture

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Hu Xianqiao, He Qiaohong, Bai Zeqing, Su Faming, Chen Hengwu. An Approach for Irreversible Bonding of PDMS-PS Hybrid Microfluidic Chips at Room Temperature[J]. Acta Chimica Sinica, 2013, 71(11): 1535-1539.

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聚二甲基硅氧烷-聚苯乙烯复合微流控芯片室温不可逆封合法的研究

An Approach for Irreversible Bonding of PDMS-PS Hybrid Microfluidic Chips at Room Temperature

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