A Simple Fabricated Microfluidic Chip for Rapid Capture of Circulating Tumor Cells

doi:10.6023/A13080902

Acta Chimica Sinica ›› 2014, Vol. 72 ›› Issue (1): 69-74.DOI: 10.6023/A13080902 Previous Articles Next Articles

Article

简易型微流控芯片捕获循环肿瘤细胞的研究

高菊逸^a,b, 杜晶辉^a,b, 张望^a,b, 张宝月^c, 刘宗彬^c, 陈艳^c, 徐小平^b

a 南昌大学研究生院医学部南昌 330006;
b 香港大学深圳医院深圳 518053;
c 中国科学院深圳先进技术研究院深圳 518055

投稿日期:2013-08-31 发布日期:2013-10-30
通讯作者: 徐小平，E-mail：jyoxu@163.com；Tel.：13828787979 E-mail:jyoxu@163.com
基金资助:
项目受深圳发展改革委员会项目[（2012）385]；国家自然科学基金（No. 61106128）和深圳市科技计划项目（No. 201101004）资助.

A Simple Fabricated Microfluidic Chip for Rapid Capture of Circulating Tumor Cells

Gao Juyi^a,b, Du Jinghui^a,b, Zhang Wang^a,b, Zhang Baoyue^c, Liu Zongbin^c, Chen Yan^c, Xu Xiaoping^b

a Medical Department of Nanchang University Graduate School, Nanchang 330006;
b Hong Kong University Shenzhen Hospital, Shenzhen 518053;
c Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055

Received:2013-08-31 Published:2013-10-30
Supported by:
Project supported by the Shenzhen Development and Reform Commission Project (Grant 2012-385), the National Natural Science Foundation of China (No. 61106128), Shenzhen Science and Technology Plan Project (No. 201101004).

1. A Simple Fabricated Microfluidic Chip for Rapid Capture of Circulating Tumor Cells.PDF(563KB)

Abstract

Due to their portability, affordability and high sensitivity, microfluidic systems have become promising technologies to develop point-of-care (POC) diagnostics. A major challenge in microfluidic system fabrication is to integrate multiple microfluidic components in a simple way and construct microfluidic systems in a rapid, inexpensive manner. Here we investigated the design and fabrication of rapid capture of circulating tumor cells (CTCs) without the need for a cleanroom facility. The device contains is composed of a polydimethylsiloxane (PDMS) layer and a double-sided adhesive film (DSA). The microfluidic channels on the DSA film were simply fabricated using a laser prototyping technique. PDMS was chosen as the support layer to facilitate sample injection and avoid leakage. The microfluidic chip fabrication takes less than one minute without employing expensive lithographic methods. Design changes of fluidic channel can be made by ProE software, and which is applicable to rapid prototyping of microfluidic devices. The PDMS base and a glass cover slip were then assembled via the DSA. Before assembling the chip, glass cover was cleaned with ethanol using sonication. Then, it was washed with distilled water and dried under nitrogen gas. After cleaning steps, the glass cover was plasma treated for 60 seconds (100 mW, 1% O₂, 60 s). Next, specific antibody was pre-immobilized on the bottom of the microchannel and then bound with FITC-labeled detection antibodies to generate fluorescent signals. Then, the inlet with cell suspension solution was connected to a syringe pump which controlled the fluid velocity, flow rates was adjusted from 2 to 8 μL/min. Using this device, a high tumor cell capture efficiency of 92% was achieved at a flow velocity of 2 μL/min. In this paper, a new technique is described to reduce fabrication time and cost, and shows considerable promise for the translation to a rapid point-of-care diagnostic device for the detection of CTCs.

Key words: microfluidic chip, capture, circulating tumor cells, simple, double-sided adhesive film

Cite this article

Gao Juyi, Du Jinghui, Zhang Wang, Zhang Baoyue, Liu Zongbin, Chen Yan, Xu Xiaoping. A Simple Fabricated Microfluidic Chip for Rapid Capture of Circulating Tumor Cells[J]. Acta Chimica Sinica, 2014, 72(1): 69-74.

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简易型微流控芯片捕获循环肿瘤细胞的研究

A Simple Fabricated Microfluidic Chip for Rapid Capture of Circulating Tumor Cells

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