Chin. J. Org. Chem. ›› 2018, Vol. 38 ›› Issue (9): 2400-2411.DOI: 10.6023/cjoc201804046 Previous Articles     Next Articles

Special Issue: 合成科学

Reviews

赖氨酸翻译后修饰蛋白质的化学(半)合成及其应用

王志鹏a,b, 李曼c, 李珲d, 刘志华c, 李影a, 郑基深a   

  1. a 中国科学技术大学生命学院 合肥 230027 中国;
    b 德州农工大学化学系 得克萨斯州 77840 美国;
    c 德州农工大学植物病理与微生物学系 得克萨斯州 77843 美国;
    d 安徽省兽药饲料监察所 合肥 230091 中国
  • 收稿日期:2018-04-28 修回日期:2018-05-19 出版日期:2018-09-25 发布日期:2018-06-07
  • 通讯作者: 郑基深, 王志鹏 E-mail:jszheng@ustc.edu.cn;zhipeng.wang@chem.tamu.edu
  • 基金资助:

    国家自然科学基金(No.U1732161)、安徽省杰出青年科学基金(No.1808085J04)和合肥物质科学技术中心方向项目培育基金(No.2017FXCX002)资助项目.

Chemical (Semi-) Synthesis and Applications of Lysine Post-Translationally Modified Proteins

Wang Zhipenga,b, Li Manc, Li Huid, Liu Zhihuac, Li Yinga, Zheng Ji-Shena   

  1. a School of Life Sciences, University of Science and Technology of China, Hefei 230027, China;
    b Department of Chemistry, Texas A & M University, Texas 77840, USA;
    c Department of Plant Pathology and Microbiology, Texas A & M University, Texas 77843, USA;
    d Anhui Supervision of Veterinay Drug and Feed, Hefei 230091, China
  • Received:2018-04-28 Revised:2018-05-19 Online:2018-09-25 Published:2018-06-07
  • Contact: 10.6023/cjoc201804046 E-mail:jszheng@ustc.edu.cn;zhipeng.wang@chem.tamu.edu
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. U1732161), the Science and Technological Fund of Anhui Province for Outstanding Youth (No. 1808085J04) and the Innovative Program Development Foundation of Hefei Center Physical Science and Technology (No. 2017FXCX002).

The study of native proteins with post-translational modifications (PTMs) is one of the main fields of epigenetics. The discovery of novel PTM models and their vital regulatory role for chromatin structure and gene transcription have been one of the current research focuses drawing attention of biologists especially in recent years. However, we still lack efficient strategies for the preparation of sufficient amount of native proteins with certain PTMs. The currently existing chemical biology methods are reviewed, and their advantages and disadvantages are compared, including bioorthogonal reaction technique, non-canonical amino acid incorporation, etc. Furthermore, the draft will mainly focus on the application of bioorthogonal reactions on unnatural functional groups for the incorporation of lysine PTMs.

Key words: chemical synthesis of proteins, post-translational modifications, non-canonical amino acid, bioorthogonal reaction, proteome