Chinese Journal of Organic Chemistry ›› 2020, Vol. 40 ›› Issue (5): 1275-1280.DOI: 10.6023/cjoc201911040 Previous Articles     Next Articles

软珊瑚共附生真菌Aspergillus sp.EGF15-0-3的柳珊瑚酸类代谢产物研究

冯婵a, 韦霞a, 胡金姗a, 王思玉a, 刘炳新a, 谢珍玉b, 荣莉a, 李小翚a, 张翠仙a   

  1. a 广州中医药大学中药学院 广州 510006;
    b 海南大学药学院 海口 570228
  • 收稿日期:2019-11-30 修回日期:2020-01-13 发布日期:2020-02-15
  • 通讯作者: 李小翚, 张翠仙;
  • 基金资助:

Researches on the Subergane-Type Sesquiterpenes from the Soft Coral-Derived Fungus Aspergillus sp. EGF15-0-3

Feng Chana, Wei Xiaa, Hu Jinshana, Wang Siyua, Liu Bingxina, Xie Zhenyub, Rong Lia, Li Xiaohuia, Zhang Cuixiana   

  1. a School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006;
    b College of Marine Science, Hainan University, Haikou 570228
  • Received:2019-11-30 Revised:2020-01-13 Published:2020-02-15
  • Supported by:
    Project supported by the National Natural Science Foundation of China (No.81741160), the Natural Science Foundation of Guangdong Province (No. 2014A030313411), the Science and Technology Planning Project of Guangdong Province (Nos. 2017A020217008, 2015A020216017), the Special Project for Marine Economic Development (Six Major Marine Industries) of Guangdong Province of Department of Natural Resources of Guangdong Province (No. [2020]039), the Open Subject of Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, the Fire Plan Project of the Guangzhou University of Chinese Medicine (No. XH20170110), and the Open Subject of State Key Laboratory of Marine Resources Utilization in the South China Sea of Hainan University (No. 2016003).

Subergorgic acid (1), a cyclopentapentalane sesquiterpenoid and potential reversible cholinesterase inhibitor, and its analogue 2-deoxy-2β-hydroxysubergorgic acid (2) were first isolated from soft coral-associated symbiotic and epiphytic fungus Aspergillus sp. EGF15-0-3. Their structures were established by NMR and HRESIMS spectroscopic data. According to the strategy of one strain many compounds (OSMAC) and eutrophic culture, Global Natural Products Molecular Networking (GNPS) was applied to detect the presence of compounds 1 and 2 in 23 different media for optimal medium compositions. As a result, compound 1 was found in 6 different media besides potato dextrose broth (PDB) medium, the contents of 2 were different in the solid and liquid media, and the best medium for compounds 1 and 2 was GPY+CaCO3. It was demonstrated that the soft coral-associated symbiotic and epiphytic fungus Aspergillus sp. EGF15-0-3 was the microbiological producer of compounds 1 and 2. This work provided strong evidence to support the hypothesis that novel and bioactive secondary metabolites produced by marine invertebrates often come from endogenetic, epiphytic, symbiotic or environmental microorganisms.

Key words: soft coral-associated symbiotic and epiphytic fungus, Aspergillus sp.EGF15-0-3, subergorgic acid, GNPS