A Ratiometric Fluorescent Probe for Imaging Hydrogen Peroxide in Living Cells

doi:10.6023/cjoc202002030

Chinese Journal of Organic Chemistry ›› 2020, Vol. 40 ›› Issue (9): 2888-2894.DOI: 10.6023/cjoc202002030 Previous Articles Next Articles

一种比率型荧光探针用于成像活细胞中的过氧化氢

杨敏^a, 夏丽丽^b, 周小琴^a, 贾程利^a, 吉民^a, 王鹏^b

a 东南大学生物科学与医学工程学院南京 210009;
b 中国药科大学工学院南京 210009

收稿日期:2020-02-23 修回日期:2020-05-18 发布日期:2020-06-13
通讯作者: 王鹏, 吉民 E-mail:wangpeng@cpu.edu.cn;101010516@seu.edu.cn
基金资助:
国家自然科学基金面上（No.81671745）资助项目.

A Ratiometric Fluorescent Probe for Imaging Hydrogen Peroxide in Living Cells

Yang Min^a, Xia Lili^b, Zhou Xiaoqin^a, Jia Chengli^a, Ji Min^a, Wang Peng^b

a School of Biological Sciences and Medical Engineering, Southeast University, Nanjing 210009;
b Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing 210009

Received:2020-02-23 Revised:2020-05-18 Published:2020-06-13
Supported by:
Project supported by the General Program of National Natural Science Foundation of China (No. 81671745).

1. cjoc202002030.pdf(962KB)

Abstract

Hydrogen peroxide (H₂O₂) plays an important role in various physiological and pathological processes in the form of molecular signals. Thus, it is of great significance to detect hydrogen peroxide in living cells or organisms quickly, efficiently and sensitively. In this study, a novel ratiometric fluorescent probe was designed to show the ratiometric fluorescence response to hydrogen peroxide. The probe showed the advantages of good selectivity, high sensitivity, low cytotoxicity and fast reaction rate in hydrogen peroxide detection. The probe also provided excellent performance in detecting both exogenous and endogenous hydrogen peroxide in living cells.

Key words: ratiometric fluorescent probe, exogenous, endogenous, hydrogen peroxide, living cell imaging

Cite this article

Yang Min, Xia Lili, Zhou Xiaoqin, Jia Chengli, Ji Min, Wang Peng. A Ratiometric Fluorescent Probe for Imaging Hydrogen Peroxide in Living Cells[J]. Chinese Journal of Organic Chemistry, 2020, 40(9): 2888-2894.

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一种比率型荧光探针用于成像活细胞中的过氧化氢

A Ratiometric Fluorescent Probe for Imaging Hydrogen Peroxide in Living Cells

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