Chinese Journal of Organic Chemistry ›› 2020, Vol. 40 ›› Issue (8): 2508-2512.DOI: 10.6023/cjoc202004037 Previous Articles     Next Articles

一个基于菲咯啉的Pb(II)荧光探针

梅辉辉a, 杨铭阳b, 刘晓燕a, 田玉平a, 徐括喜a   

  1. a 河南大学化学化工学院 河南开封 475004;
    b 河南大学民生学院 河南开封 475004
  • 收稿日期:2020-04-24 修回日期:2020-05-17 出版日期:2020-08-25 发布日期:2020-06-01
  • 通讯作者: 田玉平, 徐括喜 E-mail:tianyuping@henu.edu.cn;xukx@henu.edu.cn
  • 基金资助:
    国家自然科学基金(No.31872311)资助项目.

A Novel Phenanthroline-Based Fluorescent Probe for Pb(II)

Mei Huihuia, Yang Mingyangb, Liu Xiaoyana, Tian Yupinga, Xu Kuoxia   

  1. a College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004;
    b Mingsheng College, Henan University, Kaifeng, Henan 475004
  • Received:2020-04-24 Revised:2020-05-17 Online:2020-08-25 Published:2020-06-01
  • Supported by:
    Project supported by the Natural National Science Foundation of China (No. 31872311).

A novel fluorescent probe L for Pb2+ based phenanthroline derivative has been designed and synthesized. The probe L displayed fluorescence quenching emission excellent sensitivity specific toward Pb2+ ion. The introduction of Pb2+ resulted the quenching fluorescence of probe L and the color of the solution can be observed changes from yellow to orange in DMF-H2O (V:V=1:1, 10 mmol/L Tris, pH=7.4). The detection limit was as low as 2.68×10-7 mol/L. The bonding mechanism was confirmed by UV-vis, fluorescence, HRMS, 1H NMR experiments and theoretical calculation. It was found that the probe had excellent reversibility for detection of Pb2+ and could be used to effectively identify Pb2+ in living cells.

Key words: phenanthroline derivative, fluorescent probe for Pb2+, living cell imaging