Chinese Journal of Organic Chemistry ›› 2020, Vol. 40 ›› Issue (9): 2888-2894.DOI: 10.6023/cjoc202002030 Previous Articles     Next Articles


杨敏a, 夏丽丽b, 周小琴a, 贾程利a, 吉民a, 王鹏b   

  1. a 东南大学生物科学与医学工程学院 南京 210009;
    b 中国药科大学工学院 南京 210009
  • 收稿日期:2020-02-23 修回日期:2020-05-18 发布日期:2020-06-13
  • 通讯作者: 王鹏, 吉民;
  • 基金资助:

A Ratiometric Fluorescent Probe for Imaging Hydrogen Peroxide in Living Cells

Yang Mina, Xia Lilib, Zhou Xiaoqina, Jia Chenglia, Ji Mina, Wang Pengb   

  1. a School of Biological Sciences and Medical Engineering, Southeast University, Nanjing 210009;
    b Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing 210009
  • Received:2020-02-23 Revised:2020-05-18 Published:2020-06-13
  • Supported by:
    Project supported by the General Program of National Natural Science Foundation of China (No. 81671745).

Hydrogen peroxide (H2O2) plays an important role in various physiological and pathological processes in the form of molecular signals. Thus, it is of great significance to detect hydrogen peroxide in living cells or organisms quickly, efficiently and sensitively. In this study, a novel ratiometric fluorescent probe was designed to show the ratiometric fluorescence response to hydrogen peroxide. The probe showed the advantages of good selectivity, high sensitivity, low cytotoxicity and fast reaction rate in hydrogen peroxide detection. The probe also provided excellent performance in detecting both exogenous and endogenous hydrogen peroxide in living cells.

Key words: ratiometric fluorescent probe, exogenous, endogenous, hydrogen peroxide, living cell imaging