Chin. J. Org. Chem. ›› 1997, Vol. 17 ›› Issue (1): 20-23. Previous Articles     Next Articles

Original Articles

应用非天然氨基酸研究腺苷酸激酶的作用机理

ZHONG ZHAO;XIAOHONG LIU;ZHENGTAO SHI;BAOHUA HUANG;RU-TAI JIANG;蔡明道   

  1. 俄亥俄州大学化学与生化系
  • 发布日期:1997-02-25

Studying the mechanism of adenylate kinase with unnatural amino acids

ZHONG ZHAO;XIAOHONG LIU;ZHENGTAO SHI;BAOHUA HUANG;RU-TAI JIANG;CAI MINGDAO   

  • Published:1997-02-25

Unnatural amino acid site-directed mutagenesis was applied to adenylate kinase to study its mechanism. One phenylalanine and several proline unnatural amino acid analogs were specifically incorporated into adenylate kinase. Study-state kinetic studies were carried out for the resulting mutants.

Key words: TYROSINE, ADENYLIC ACID, KINASE, ACTION MECHANISM, PROLINE, PHENYLALANINE, AMINO ACID

CLC Number: