谱图检索结合二级质谱定性分析烟草中挥发性成分

doi:10.6023/A1105024

化学学报 ›› 2012, Vol. 0 ›› Issue (05): 649-658 .DOI: 10.6023/A1105024 上一篇下一篇

研究论文

谱图检索结合二级质谱定性分析烟草中挥发性成分

吴丽君^a, 段佳^a, 李倩倩^a, 曹金莉^b, 刘玮^a, 闵顺耕^a

a 中国农业大学理学院应用化学系北京 100193;
b 云南省烟草公司大理州公司云南 671000

投稿日期:2011-05-02 修回日期:2011-10-10 发布日期:2011-11-28
通讯作者: 闵顺耕 E-mail:minsg@263.net
基金资助:
云南省烟草公司(No. 09YN016)资助项目.

Identification of the Volatile Components in Tobacco by Gas Chromatograph Coupled with Ion Trap Tandem Mass Spectrometry

Wu Lijun^a, Duan Jia^a, Li Qianqian^a, Cao Jinli^b, Liu Wei^a, Min Shungeng^a

a Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193;
b Tobacco Corporation of Yunnan Dali, Yunnan 671000

Received:2011-05-02 Revised:2011-10-10 Published:2011-11-28
Supported by:
Project supported by Tobacco Corporation of Yunnan Dali (No. 09YN016).

文章分享

摘要/Abstract

采用同时蒸馏萃取法提取烟叶中的挥发性成分, 利用气相色谱-质谱联用仪(GC-MS)分离测定, 通过谱库检索和匹配度定性结合色谱保留指数方法鉴定烟叶中挥发性成分, 并引入离子阱二级质谱对谱库检索匹配度差距小、含量低,背景干扰大的物质准确定性. 采用谱图检索结合二级质谱定性共鉴定144 个化合物, 其中104 个化合物在烟草挥发性成分的文献中已有报道, 报道中有9 个化合物是通过二级质谱定性, 其余40 个化合物还未见报道. 结果表明, 离子阱二级质谱定性的引入提高了对未知化合物定性的准确性和可靠性, 适合于烟叶这类复杂植物体系的化学组分研究.

关键词: 同时蒸馏萃取, 烟叶, 挥发性成分, 离子阱二级质谱

The volatile components in tobacco leaves were extracted by simultaneous distillation and extraction (SDE), and analyzed by gas chromatography-mass spectrometry (GC-MS). The volatile components in tobacco leaves were identified by library searching and spectral matching combined with retention index. Ion trap tandem mass spectrometry was introduced to identify some components which had tiny difference of library matching, low concentration, and complex matrix. 144 components were identified by library searching, spectral matching and ion trap tandem mass spectrometry in this paper. And 104 volatile components have been reported, 9 of them were identified by ion trap tandem mass spectrometry, the other 40 components have not been reported in the literature. So ion trap tandem mass spectrometry is the precise and reliable method to identify some unknown components, and is suitable for separation and identification of complex system.

Key words: simultaneous distillation and extraction, tobacco, volatile components, ion trap tandem mass spectrometry

引用此文

吴丽君, 段佳, 李倩倩, 曹金莉, 刘玮, 闵顺耕. 谱图检索结合二级质谱定性分析烟草中挥发性成分[J]. 化学学报, 2012, 0(05): 649-658 .

Wu Lijun, Duan Jia, Li Qianqian, Cao Jinli, Liu Wei, Min Shungeng. Identification of the Volatile Components in Tobacco by Gas Chromatograph Coupled with Ion Trap Tandem Mass Spectrometry[J]. Acta Chimica Sinica, 2012, 0(05): 649-658 .

导出引用 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

分享此文

参考文献

1 Jing, Y.-Q.; Gong, C.-R.; Zhang, Y.-H. Chin. Tobacco Sci.2005, (2), 44 (in Chinese). (景延秋, 宫长荣, 张月华, 中国烟草科学, 2005, (2), 44.)

2 Huang, L.-F.; Wu, M.-J.; Sun, X.-J.; Zhong, K.-J.; Guo, Z.-M.; Dai, Y.-H.; Huang, K.-L.; Guo, F.-Q. J. Central South Univ. Technol. 2007, 14, 504.

3 Sun, K.-J.; Wang, M.-L.; Shen, Y.; Liu, B.-Z. Chin. Tobacco Sci. 2007, 28(2), 23 (in Chinese). (孙凯健, 王美琳, 沈轶, 刘百战, 中国烟草科学, 2007,28(2), 23.)

4 Irvine, W. J.; Saxby, M. J. Phytochemistry 1969, 8, 473

5 Salom′e, T.; Ad′elio, M.; Arminda, A.; L′ucia, S. Anal. Chim. Acta 2007, 584, 439.

6 Zhu, X.-L.; Gao, Y.; Chen, Z.-Y.; Su, Q.-D. Chromatographia2009, 69, 735.

7 Zagustina, N. A.; Krikunova, N. I.; Kulikova, A. K.; Misharina, T. A.; Romanov, M. E.; Ruzhitsky, A. O.; Terenina, M. B.; Veprizky, A. V.; Zhukov, V. G.; Popova, V. O. J. Chem. Technol. Biotechnol. 2010, 85, 320.

8 Fu, Y.-X.; Xu, Y.-J.; Chen, B.; Li, Y.; Luo, L.-P. Chin. J. Anal. Chem. 2009, 37(5), 745 (in Chinese). (付宇新, 徐元君, 陈滨, 丽艳, 罗丽萍, 分析化学, 2009,37(5), 745.)

9 John, E.; Bunch, T.; Jeffrey, C. J. Agric. Food Chem. 1997,45, 844.

10 Sun, S.-H.; Xie, J.-P.; Xie, F.-W.; Zong, L.-Y.; Xie, F.-W. J. Chromatogr. A 2008, 1179, 89.

11 Huang, C.-X.; Wang, Z.-W.; Liu, Y.; Wang, S.-F. Chin. J. Anal. Chem. 2008, 36, 1396 (in Chinese). (黄崇杏, 王志伟, 刘杨, 王双飞, 分析化学, 2008, 36,1396.)

12 Xu, W.-L.; Zhao, Y.-R.; Wang, W.-L. Chem. Res. 2006, 17, 92 (in Chinese). (徐伟玲, 赵云荣, 王文领, 化学研究, 2006, 17, 92.)

13 Li, X.-F.; Duan, Q.-Y. Sciencepaper Online 2008, 3, 672 (in Chinese). (李小福, 殷全玉, 中国科技论文在线, 2008, 3, 672.)

14 Zhao, M.-Q. Cigarette Perfumer Science, Science Press, Beijing, 2008, pp. 72～73 (in Chinese). (赵铭钦, 卷烟调香学, 科学出版社, 北京, 2008, pp. 72～73.)

15 Li, H.-F.; Zhong, K.-J.; Lu, X.; Bai, C.-M.; Huang, J.-G.; Lu, H.-L.; Ma, C.-F.; Zhu, S.-K.; Kong, H.-W.; Zhao, M.-Y.; Xie, J.-P.; Niu, S.; Xu, G.-W. Acta Chim. Sinica2006, 64, 1897 (in Chinese). (李海锋, 钟科军, 路鑫, 白长敏, 黄建国, 鹿洪亮, 马晨菲, 朱书奎, 孔宏伟, 赵明月, 谢剑平, 牛森, 许国旺, 化学学报, 2006, 64, 1897.)

16 Su, Y.; Liao, Y.-X.; Guo, Y.-L. Acta Chim. Sinica 2007,65(14), 1377 (in Chinese). (苏越, 廖远熹, 郭寅龙, 化学学报, 2007, 65(14), 1377.)

17 Li, Y.; Pang, T.; Guo, Z.-M.; Li, Y.-L.; Wang, X.-L.; Deng, J.-H.; Zhong, K.-J.; Lu, X.; Xu, G.-W. Talanta 2010, 81,650.

18 Huang, L.-F.; Zhong, K.-J.; Sun, X.-J.; Wu, M.-J.; Huang, K.-L.; Liang, Y.-Z.; Guo, F.-Q.; Li, Y.-W. Anal. Chim. Acta2006, 575, 236.

19 Peng, F.-M.; Sheng, L.-Q.; Liu, B.-Z.; Tong, H.-W.; Liu, S.-M. J. Chromatogr. A 2004, 1040, 1.

20 Liang, S.; Li, Y.-W.; Zhao, C.-X.; Liang, Y.-Z. J. Instrument. Anal. 2008, 27, 84 (in Chinese). (梁晟, 李雅文, 赵晨曦, 梁逸曾, 分析测试学报, 2008,27, 84.)

21 Wang, C.-Z.; Su, Y.; Guo, Y.-L. Chin. J. Org. Chem. 2009,29, 948 (in Chinese). (王呈仲, 苏越, 郭寅龙, 有机化学, 2009, 29, 948.)

22 Liu, S.-H.; Lu, S.-S.; Su, Y.; Guo, Y.-L. Chromatographia2011, 74, 497.

23 Wang, C.-Z.; Su, Y.; Wang, H.-Y.; Guo, Y.-L. J. Am. Soc. Mass Spectrom. 2011, 22, 1839.

24 Wang, H.-Y.; Guo, Y.-L. Anal. Lett. 2004, 37(10), 2151.

25 Xu, Y.-J.; Bai, C.-M.; Zhong, K.-J.; Huang, J.-G.; Tang, W.-Y.; Lu, X.; Xu, G.-W. Chin. J. Anal. Chem. 2006, 34, 382 (in Chinese). (许燕娟, 白长敏, 钟科军, 黄建国, 唐婉莹, 路鑫, 许国旺, 分析化学, 2006, 34, 382.)

[1]	陈房姣, 苏越, 郭寅龙. 后目标分析法用于盐益智仁挥发性成分的顶空-气相色谱-四级杆/飞行时间质谱分析[J]. 化学学报, 2014, 72(1): 95-104.
[2]	郭寅龙,王呈仲,苏越. 基于准确质量测定和保留指数的GC-MS分析薄荷挥发性成分[J]. 化学学报, 2009, 67(6): 546-554.
[3]	李海锋, 钟科军, 路鑫, 白长敏, 黄建国, 鹿洪亮, 马晨菲, 朱书奎, 孔宏伟, 赵明月, 谢剑平. 全二维气相色谱/飞行时间质谱(GC×GC/TOFMS)用于烟叶中挥发、半挥发性碱性化合物的组成研究[J]. 化学学报, 2006, 64(18): 1897-1903.

谱图检索结合二级质谱定性分析烟草中挥发性成分

Identification of the Volatile Components in Tobacco by Gas Chromatograph Coupled with Ion Trap Tandem Mass Spectrometry

PDF

可视化

被引次数

摘要/Abstract

引用此文

分享此文

参考文献

相关文章 3

编辑推荐

相关统计

本文评价