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化学学报
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化学学报 ›› 2012, Vol. 0 ›› Issue (04): 459-464.DOI: 10.6023/A1106303 上一篇    下一篇

研究论文

HPLC-DAD 结合交替三线性分解二阶校正方法进行中药葛根中主要有效成分同时测定

苏志义, 吴海龙, 刘亚娟, 许慧, 张娟, 聂重重, 俞汝勤   

  1. 湖南大学化学化工学院 化学生物传感与计量学国家重点实验室 长沙 410082
  • 投稿日期:2011-06-30 修回日期:2011-10-10 发布日期:2011-10-25
  • 通讯作者: 吴海龙
  • 基金资助:

    国家自然科学基金(Nos. 20775025, 21175041)资助项目.

Simultaneous Determination of Main Effective Constituents in Traditional Chinese Medicine Kudzuvine Root Using HPLC-DAD Coupled with Second-order Calibration Based on Alternating Trilinear Decomposition

Su Zhiyi, Wu Hailong, Liu Yajuan, Xu Hui, Zhang Juan, Nie Chongchong, Yu Ruqin   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082
  • Received:2011-06-30 Revised:2011-10-10 Published:2011-10-25
  • Supported by:

    Project was supported by the National Natural Science Foundation of China (Nos. 20775025, 21175041).

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应用高效液相色谱-二极管阵列联用仪(HPLC-DAD)结合基于交替三线性分解(ATLD)算法的二阶校正方法快速测定了中药葛根样中主要活性成分葛根素、大豆苷和大豆苷元的含量, 实现了同时定量分析. 色谱条件: 甲醇-水(体积比为53∶47), 检测波长范围为190～380 nm, 柱温为30 ℃, 流速为1.0 mL /min, 进样量为20.0 μL. 预测的实际样中三种目标分析组分葛根素、大豆苷、大豆苷元的含量分别为(0.465±0.023), (0.553±0.015)和(0.098±0.005) mg/g, 它们的加标回收率分别为(101.1±3.2)%, (100.4±6.4)%和(100.1±4.9)%.

关键词: 二阶校正, 交替三线性分解, HPLC-DAD, 葛根, 葛根素, 大豆苷, 大豆苷元

A method for simultaneously quantifying the main effective constituents such as puerarin, daidzin and daidzein in traditional Chinese medicine kudzuvine root was developed in this paper, by using high performance liquid chromatography coupled with diode array detection (HPLC-DAD) and secondorder calibration method based on alternating trilinear decomposition (ATLD) algorithm. Profiting from the application of second-order calibration method, it needs very simple chromatographic conditions. The mobile phase consisted of methanol (53%, φ) and water (47%, φ), which was pumped at a flow rate of 1.0 mL/min with 20.0 μL injection volume. The column temperature was set at 30 ℃. The range of scanning wavelengths were 190～380 nm. The predicted contents of puerarin, daidzin and daidzein in kudzuvine root samples were (0.465±0.023), (0.553±0.015) and (0.098±0.005) mg/g, respectively and their spiked recoveries were (101.3±3.2)%, (100.4±6.4)% and (100.1±4.9)%, respectively.

Key words: second-order calibration, ATLD, HPLC-DAD, kudzuvine root, puerarin, daidzin, daidzein