化学学报 ›› 2012, Vol. 70 ›› Issue (06): 714-720.DOI: 10.6023/A1107051 上一篇    下一篇

研究论文

甲醇-水混合溶剂后处理制备丝素蛋白膜及其药物释放研究

韦俏娜, 马林, 黄爱民, 杨华, 龚珠萍, 强盼盼, 张丽   

  1. 广西大学化学化工学院 南宁 530004
  • 投稿日期:2011-07-05 修回日期:2011-11-16 发布日期:2011-12-09
  • 通讯作者: 马林
  • 基金资助:

    国家自然科学基金(No. 21063002), 广西自然科学基金(No. 2011jjC30004)和广西研究生创新计划(No. 105931003028)资助项目.

Controlled Drug Release from Silk Fibroin Films Treated with Methanol-water Mixtures

Wei Qiaona, Ma Lin, Huang Aimin, Yang Hua, Gong Zhuping, Qiang Panpan, Zhang Li   

  1. College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004
  • Received:2011-07-05 Revised:2011-11-16 Published:2011-12-09
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 21063002), Natural Science Foundation of Guangxi Province (No. 2011jjC30004) and Post-graduate Innovation Program of Guangxi Province (No. 105931003028).

采用溶液浇注法制备丝素蛋白薄膜, 应用傅里叶红外光谱(FTIR)和X 射线衍射(XRD)研究了浓度不同的甲醇-水混合溶剂处理后丝素蛋白薄膜的结构变化, 并以罗丹明B 为模型药物与丝素蛋白构建药物缓释体系, 考察了丝素蛋白膜的结晶结构对药物释放动力学的影响. 结果显示, 在甲醇体积比浓度ΦMeOH=50%~90%的范围内, 丝素蛋白材料中以β-折叠为主的silk Ⅱ 结晶含量随着混合溶剂中甲醇浓度的增加而先增加后下降, 在ΦMeOH=80%附近出现最大值. 罗丹明B 从丝素蛋白膜的释放属于Fickian 扩散机理, 其扩散指数n 随着丝素蛋白膜中β-折叠含量的增加而增加, silk Ⅱ结晶是丝素蛋白材料药物释放的天然调节器.

关键词: 丝素蛋白, 药物缓释, 结构变化

Silk fibroin (SF) films were prepared using a casting method and the structural change owing to treatment with methanol-water mixtures of different concentrations was investigated by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The results were used to reveal the influence of structure on the release dynamics of Rhodamine B, a model compound loaded in the SF matrix. In methanol concentration ranging from 50% to 90%, the content of silk Ⅱ crystalline structure, constituted by β-sheet, first increased and then decreased with increasing methanol concentration in the mixtures, reaching a maximum around 80%. It was found that Rhodamine B released from the SF film via Fickian mechanism, of which the diffusional exponent increased with increasing β-sheet content in the matrix, suggesting that the silk Ⅱ crystal could be used as natural regulator for drug release from SF material.

Key words: silk fibroin, controlled release, structural change