关键词: 荧光偏振, 蛋白激酶A, 磷酸化, TiO₂纳米棒, 抑制剂筛选

Protein phosphorylation by protein kinase plays a critical role in the process of cellular signal transduction, which is closely associated with many fundamental biological processes including cell growth and apoptosis. Aberrant states of protein kinase activities are involved in many diseases, such as diabetes, Alzheimer's disease and cancers. Moreover, protein kinase has now become one of the most important groups of drug targets. The screening of protein kinase inhibitors is becoming increasingly important in the targeted therapy. Therefore, rapid and sensitive detection of protein kinase activity is of great significance for better understanding the life process, clinical diagnosis as well as discovery of new targeted drugs. In this study, a simple, ultrasensitive and cost-effective assay is developed for detection of protein kinase activity by using fluorescence polarization technique based on the specific recognition ability of TiO₂ nanorods for kinase-induced fluorescent phosphopeptides. In the presence of protein kinase, the fluorescence-labeled substrate peptides can be phosphorylated with the catalysis of the protein kinase. Then the phosphopeptides can specifically bind to Ti⁴⁺ on the surface of the TiO₂ nanorods by means of phosphate groups, leading to remarkable change of rotation speed of the fluorophores, which subsequently results in the great increase of fluorescence polarization signal. By monitoring the fluorescence polarization signals, protein kinase A（PKA), a proof-of-concept kinase target, can be detected in the range of 0.0005～1.0 U·μL^-1 and the detection limit is estimated to be 0.0004 U·μL^-1, indicating that the proposed assay is one of the most sensitive assays for PKA activity detection. Furthermore, the proposed assay is also sucessfully applied to PKA inhibition assay by using H-89 as the model inhibitor of PKA. The IC₅₀ value (inhibitor concentration producing 50% inhibition) is determined as 135 nmol·L^-1, which is well consistent with that reported in the literatures. For the proposed assay, the TiO₂ nanorods are commercially available with low cost and can be used without any modification. The fluorescence polarization can be directly measured in the homogeneous solution. Therefore, the new strategy provides a simple detection procedure, easy readout and cost-effective manner for protein kinase assay.

Key words: fluorescence polarization, protein kinase A, phosphorylation, TiO₂ nanorods, screening of inhibitor