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化学学报
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化学学报 ›› 1966, Vol. 32 ›› Issue (3): 284-291. 上一篇    

研究简报

C14-标记牛胰岛素A链和C14-标记牛胰岛素的合成

汪猷1, 钱瑞卿1, 张伟君1, 杜雨蒼2, 沈昭文2, 钮经义2, 季爱雪3, 施溥涛3, 邢其毅3   

  1. 1. 中国科学院有机化学研究所;
    2. 中国科学院生物化学研究所;
    3. 北京大学化学系
  • 投稿日期:1966-03-30 发布日期:2013-06-03

SYNTHESIS OF C14-LABELED A-CHAIN OF BOVINE INSULIN AND C14-LABELED BOVINE INSULIN

WANG YU1, CHIEN RAY-CHING1, CHANG WEI-CHUN1, DU YU-CANG2, SHEN CHAO-WEN2, NIU CHING-I2, CHI AI-HSUEH3, SHI PU-TAO3, HSING CHI-YI3   

  1. 1. Institute of Organic Chemistry, Academia Sinica;
    2. Institute of Biochemistry, Academia Simca;
    3. Department of Chemistry, Peking University
  • Received:1966-03-30 Published:2013-06-03

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根据前文叙述的方法,我们用[1-C14]-甘氨酸制备了带保护基的牛胰岛素A链氨端五肽Ⅰ(Z-1-C14-Gly-Ile-Val-Glu(OBut)-Glu(NH2)-NHNHBOC)和氨端九肽Ⅱ(Z-1-C14-Gly-Ile-Val-Glu-Glu(NH2)-Cys(BZL)-Cys(BZL)-Ala-Ser(OAc)-NHNH2)。

By the use of carbobenzoxy-l-C14-glycine (47.6μc/mg) we have synthesized according to our procedure for the preparation of non-isotopic insulin peptides[1] labeled pentapeptide Ⅰ (N-carbobenzoxy-l-C14-glycyl-L-isoleucyl-L-valyl-γ-t-butyl-L-glutamyl-L-glutaminyl-carbo-t-butoxyhydrazide, 11μc/mg) and labeled nonapeptide Ⅱ (N-carbobenzoxy-l-C14-glycyl-L-isoleucyl-L-valyl-L-glutamyl-L-glutaminyl-S-benzyl-L-cysteinyl-S-benzyl-L-cysteinyl-L-alanyl-L-serylhydrazide, 7.9μc/mg) (Table 1).