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化学学报
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化学学报 ›› 1981, Vol. 39 ›› Issue (4): 367-370. 上一篇    下一篇

研究简报

农药代谢产物分析的研究——Ⅱ.尿中5-氯-氨茴酸的分离与薄层分析

杜锡光1, 李定俊1, 毛志翔2, 刘志强2, 骆文莹2   

  1. 1. 浙江医科大学;
    2. 浙江医科大学农药研究室, 杭州
  • 投稿日期:1979-05-07 发布日期:2013-06-03

STUDIES ON ANALYSIS OF METABOLITES OF PESTICIDES Ⅱ. ISOLATION AND TLC OF 5-CHLOROANTHRANILIG ACID IN URINE

DU XI-GUANG1, LI DING-JUN1, MAO ZHI-XIANG2, LIU ZHI-JIANG2, LUO WEN-YING2   

  1. 1. Zhejiang Medical College, Hangzhou;
    2. Pesticide Laboratory, Zhejiang Mediccal College, Hangzhou
  • Received:1979-05-07 Published:2013-06-03

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前报[1]中曾报道尿中杀虫脒及其部分代谢产物的气相层析分析.其中5-氯-氨茴酸熔点高,须先制成甲酯,由于尿样的纯化不佳,对该化合物的定量测定误差较大.我们曾用离子交换柱法[2,3]进行尿样的纯化试验,发现文献中所报道的条件均不能满足对检测该化合物的需要.为此,我们进行了探索,找到了从尿中分离并纯化该化合物的较佳条件.

In order to find out optimal conditions for the column chromatographic isolation of 5-ohloroanthranilio acid from urine, three columns were tested. The best isolation is obtained by ion-ezahange chromatography on a column packed with Zerolite 225 (200~300 mesh),the column is eluted at 25~30 ml/hr with 80 ml 0.5M Na2HPO4 solution. Recovery of 5-chloroanthnanilio acid from urine is 76~90%.