化学学报 ›› 1992, Vol. 50 ›› Issue (6): 594-600. 上一篇    下一篇

研究论文

电致化学发光免疫分析测定人绒毛膜促进性激素

安镜如;林建国   

  1. 福州大学化学系;武汉产品质量监督检验所
  • 发布日期:1992-06-15

Investigation of electrochemiluminescence immunoassay for determination of HCG

AN JINGRU;LIN JIANGUO   

  • Published:1992-06-15

本文用电化学发光法进行了人绒毛膜促进性激素(简称HCG)的免疫分析研究。实验在Na~2CO~3-NaHCO~3-KCl介质中以CoCl~2为催化剂测定发光标记试剂ABEI, 检测限达到1.3X10^-^1^2mol.L^-^1。ABEI-HCG标记物稀释至2X10^5倍仍可检出。采用双抗法测定了血清中的HCG。

关键词: 免疫测定, 血清, 电致发光, 二元酮, 吩噻嗪 P, 氯化钴, 人体, ABE1, 绒毛促进性激素

The electrochemiluminescence immunoassay of HCG in a medium of Na2CO3-NaHCO3-KCl-CoCl2-ABEI was studied. The luminescent reagent signed-ABEI is determine in this medium. The detection limit of this method is 1.3 ?10-12 mol譒-1 (for ABEI). This method has been successfully applied to the determination of HCG in human serum.

Key words: IMMUNOASSAY, SERUM, ELECTROLUMINESCENCE, DIKETONE, PHENOTHIAZINE P, COBALT CHLORIDE, HUMAN BODY

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