关键词: 生物活性, 尿酸酶, 生物电化学, 酶电极, 聚吡咯

The polypyrrole uricase electrode has a fast bioelectrochem. response. In the region from 5.9?0-6 to 1.5?0-3 mol譫m-3 of uric acid, the response current of the polypyrrole uricase electrode is proportional to the substrate concentration The apparent activation energy of the catalyzed reaction of the immobilized enzyme is 34.81 kL譵ol-1. As compared with the soluble uricase, advantageous changes were obtained for the immobilized enzyme in the pH and temperature dependences of the activity. The repeatability of the uricase electrode for the determination of the uric acid concentration is affected by the kind of conducting polymer, which is used for a support material of the immobilized enzyme.

Key words: BIOLOGICAL ACTIVITY, URATE OXIDASE, BIOELECTROCHEMISTRY, ENZYME ELECTRODES, POLYPYRROLE