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化学学报
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化学学报 ›› 1997, Vol. 55 ›› Issue (8): 806-810. 上一篇    下一篇

研究论文

多羟基化合物化学发光分析新体系的研究: 尿液中尿酸的测定

封满良;黄玉文;章竹君   

  1. 陕西师范大学化学系
  • 发布日期:1997-08-15

New chemiluminescence reaction system for analysis of polyhydroxy-compounds: Determination of uric acid in urine

FENG MANLIANG;HUANG YUWEN;ZHANG ZHUJUN   

  • Published:1997-08-15

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610

被引次数

16 | 3

本文首次提出使用luminol-K7[Cu(IO6)2]-KOH化学发光体系, 结合流动注射技术进行多羟基化合物的测定。方法基于多羟基化合物能增强luminol与K7[Cu(IO6)2]反应产生的化学发光, 且浓度与增强作用的大小呈线性关系。利用该法进行尿酸的测定, 发现尿酸在2×10^-^8~1×10^-^6g/mL浓度范围内与化学发光强度呈线性关系, 检出限为7.2×10^-^9g/mL, 相对标准偏差为2.04%。

关键词: 尿, 鲁米诺, 氢氧化钾, 流动注射分析, 化学发光, 脲酸, 高碘酸合铜酸钾

A novel flow injection chemiluminescence method for the determination of polyhydroxy-compounds was described. The method is based on that polyhydroxy-compounds enhance the Cl reaction of luminol with K7[Cu(IO6)2] in potassium hydroxide medium. In the experiments, luminescent reagent-luminol was injected in order to keep the baseline steady and save reagents, especially, to improve sensitivity. The method was used in measuring uric acid in urine and it allows quantitation of uric acid concentration in the 2×10^-^8~1×10^-^6g/mL range with a detection limit of 7.2×10^-^9g/mL. RSD=2.04%.

Key words: URINE, LUMINOL, POTASSIUM HYDROXIDE, FLOW INJECTION ANALYSIS, CHEMILUMINESSENCE

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