化学学报 ›› 1998, Vol. 56 ›› Issue (3): 298-301. 上一篇    下一篇

研究论文

人参皂苷的基质辅助激光解吸质谱研究

周雨;宋凤瑞;刘淑莹;李向高   

  1. 中国科学院长春应用化学研究所;吉林农业大学中药材学院
  • 发布日期:1998-03-15

Matrix-assisted laser desorption/ionization of ginsenosides

ZHOU YU;SONG FENGRUI;LIU SHUYIN;LI XIANGGAO   

  • Published:1998-03-15

利用MALDI-TOFMS测定了八种人参皂苷的分子量, 并分析了西洋参总皂苷的组成。同时, 进行了灵敏度实验, 并探讨了基质及碱金属离子的影响, 证明该方法灵敏度高, 重复性好, 结果准确。是测定极性小分子分子量的有效方法。

关键词: 分子量, 人参, 质谱法, 解吸, 激光, 皂苷

Molecular weight of 8 ginsenosides and the component of total saponions in American ginseng have been determined by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The average error of the molecular weight of each ginsenoside was found less than 0.05%. The results demonstrate that MALDI-MS is a very simple and useful method to measure the molecular weight of some high polar, thermal unstable small molecules with high sensitivity and reproducibility.

Key words: MOLECULAR WEIGHT, PANAX GINSENG, MASS SPECTROGRAPHY, DESORPTION, LASER

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