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化学学报
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化学学报 ›› 1999, Vol. 57 ›› Issue (11): 1262-1269. 上一篇    下一篇

研究论文

选择性NMR新技术用于新的3, 28-双糖链三萜皂苷的结构研究

缪振春;冯锐;周永新;魏锋   

  1. 中国军事医学科学院药理毒理研究所;北京江中药物研究所
  • 发布日期:1999-11-15

Structural determination of oligosaccharide chains of a new 3, 28- bidesmosidic triterpenoid glycoside by new selective NMR techniques

Miao Zhenchun;Feng Rui;Zhou Yongxin;Wei Feng   

  • Published:1999-11-15

PDF

477

被引次数

10

从中药川续断根部的乙醇提取物中分得一个新的三萜皂苷。经过测定,它为:3-O-[α-L-吡喃鼠李糖(1→3)][-β-D-吡喃葡萄糖(1→4)]-β-D-吡喃葡萄糖(1→3)-α-L-吡喃鼠李糖(1→2)-α-L-吡喃阿拉伯糖-常春藤苷元-28-O-β-D-吡喃葡萄糖(1→6)-β-D-吡喃葡萄糖酯苷。研究表明,采用一维SEMDY、旋转坐标NOE差谱和选择性远程DEPT核磁共振新技术相结合的方法测定糖链结构不需要对该化合物1进行化学降解或衍生化,方法简便、快速,测定结果可靠,每个糖基的信号可以分辨和明确归属。

关键词: 三萜, 川续断, 皂角甙, 核磁共振谱法, 吡喃P, 葡萄糖

A new 3, 28-bidesmosidic triterpenoid saponin was isolated from the ethanolic extract of the root of Dipsacus asperoides C. Y. cheng et T. M. Ai and its structure established as 3-O-[α-L-rhammopyranosyl (1→ 3)][-β -D-glycopranosyl (1→4)]-β-D-glycopranosyl (1→3)-α -L- rhammopyranosyl (1→2)-α -L-arabinopyranosyl-hederagenin-28-O-β-D- glycopranosyl (1→ 6)-β-D-glycopranoside. The results obtained showed that the chemical structure of oligosaccharide chains of the saponin could be elucidated by the combination of 1D-SEMDY, NOE difference spectroscopy in rotating frame and selective long-range DEPT techniques, without having recourse to chemical degradation or modification. Complete assignments of severely overlapping proton resonance of the sugar residues were made.

Key words: TRITERPENE, NMR SPECTROMETRY, PYRANE P, GLUCOSE

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