化学学报 ›› 2001, Vol. 59 ›› Issue (3): 421-425. 上一篇    下一篇

研究论文

电喷雾质谱法中K^+加合离子受Cs^+影响及在牛膝多糖分子 量分布测定中的应用

胡守刚;郭寅龙;吕龙;田庚元   

  1. 中国科学院上海有机化学研究所.上海(200032)
  • 发布日期:2001-03-15

Effect of Cs^+ on K^+ adduct ions in electrospray mass spectrometry and its application in measurement of molecular weight distribution for achyranthes bidentata polysaccharides

Hu Shougang;Guo Yinlong;Lu Long;Tian Gengyuan   

  1. Shanghai Inst Organ Chem., CAS.Shanghai(200032)
  • Published:2001-03-15

以测定牛膝多糖的分子量分布为例,研究了在Cs^+存在情况下,电喷雾(ESI)/飞行时间质谱法(TOF-MS)中出现加合离子(adductions)的规律。实验中发现,牛膝多糖样品中加入适量的Cs^+,可影响其与Na^+,K^+加合离子的相对丰度,使K^+加合离子成为几乎唯一的加合离子形式,并获得了满意结果。另外讨论了Cs^+浓度、喷管电压(nozzlepotential)对这一现象的影响。

关键词: 电喷雾离子化, 飞行时间质谱法, 牛膝多糖, 加合离子, 铯离子

Relative abundance of adduct ions was studied in measuring molecular weight distribution of achyranthes bidentata polysaccharides using electrospray ionization (ESI)/time-of -flight mass spectrometry (TOF MS) in the presence of Cs^+. The relative abundance of K^+ adduct ions, (C~6H~1~0O~5)~n+H~2O+H^++K^+, was affected by Cs^+ and become an almost unique form of adduct ions at suitable concentrations of Cs^+. This can greatly increase sensitivity and simplify the spectrum. Measuring the molecular weight distribution of achyranthes bidentata polysaccharides thus became easier, and electrospray ionization found a new application. The concentration of Cs^+ and nozzle potential were key factors that affect the relative abundance of K^+ adduct ions.

Key words: TIME-OFF FLIGHT MASS SPECTROMETRY, CESIUM ION

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