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化学学报
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化学学报 ›› 2002, Vol. 60 ›› Issue (10): 1828-1833. 上一篇    下一篇

研究论文

石墨电极固载DNA的一种新修饰方法

郑赛晶;林祥钦;尹屹梅   

  1. 中国科学技术大学化学系,合肥(230026)
  • 发布日期:2002-10-15

A New Method for Surface Modification of Paraffin Impregnated Graphite Electrode for DNA Immobilization and Hybridization

Zheng Saijing;Lin Xiangqin;Yin Yimei   

  1. Department of Chemistry, University of Science and Technology of China,Hefei(230026)
  • Published:2002-10-15

PDF

365

被引次数

20 | 4

首次报道通过充石蜡石墨电极电化学氧化腐蚀制备的微孔穴电极，成功地用于 ss-DNA的固载和杂化反应，证明了这种微孔穴石墨充石蜡电极可以固载较多量的 ss-DNA（包括解链的小牛胸腺DNA和15-mer ss-DNA），可以成为性能良好的DNA杂 化探针。用Co(phen)_3~(3+)电化学指示剂和循环伏安方法，讨论了电极预氧化腐 蚀的时间和ss-DNA固载时间的影响，提出了DNA探针在微孔穴三维结构中固载的可 能机理。

关键词: DNA, 化学修饰电极, 循环伏安法, 胸腺

A micro-hollow paraffin impregnated graphite electrode (MHPIGE) was developed by electrooxidation of a paraffin impregnated graphite electrode for immobilization of DNA. Immobilization of single string DNA from CT-DNA and 15 mer ss-DNA onto the prepared MHPIGE was investigated by cyclic voltammetry using Co(phen)_3~(3+) as an indicator. It was demonstrated that the MHPIGE could immobilize significant amounts of ss-DNA, but not ds-DNA, under mild conditions. The immobilized ss-DNA underwent hybridization reaction very well. The effects of duration on pre-oxidation treatment and on ss-DNA immobilization reaction were investigated. The mechanism of DNA immobilization on the MHPIGE is proposed as an effect of the specifically formed three-dimensional micro-hollow structure.

Key words: DNA, CHEMICAL MODIFIED ELECTRODE, CYCLOVOLTAMGRAPH, THYMUS GLAND

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