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化学学报
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化学学报 ›› 2002, Vol. 60 ›› Issue (11): 2049-2055. 上一篇    下一篇

研究论文

羧甲基牛膝多糖的制备、结构及生物活性研究

邓乐华;田庚元   

  1. 中国科学院上海有机化学研究所.上海(200032)
  • 发布日期:2002-11-15

Studies on the Preparation, Structure and Bioactivity of CM-AbPS

Deng Lehua;Tian Gengyuan   

  1. State Key Laboratory of Bio-organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences. Shanghai(200032)
  • Published:2002-11-15

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被引次数

65 | 6

在NaOH水溶液中，以ClCH_2COOH为羧甲基化试剂，对牛膝多糖(AbPS)进行了羧 甲基化，粗产物经DEAE-Cellulose及Sephadex G-25两次柱层析纯化，得到羧甲基 牛膝多糖(CM-AbPS)纯品，经高效液相法（HPLC）及毛细管电泳法（CE）检测表明 具有较好的均一性，并通过样品甲基化和GC-MS分析，对羧甲基牛膝多糖链中羧甲 基的分布进行了研究，结果表明羧甲基牛膝多糖中羧甲基主要取代的糖链中呋喃果 糖的4-位上。该产物具有抗肿瘤活性。

关键词: 牛膝, 多糖, 羧甲基, 高速液体色谱, 抗肿瘤药

Achyranthes bidentata Polysaccharides (AbPS) was carboxymethylated by ClCH_2COOH in aqueous NaOH and the crude product was purified by successive DEAE-Cellulose and Sephadex G-25 column chromatography to yield homogeneous carboxymethylated product (CM-AbPS), which was detected by high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). By the methylation method and GC-MS analysis, we studied the distribution of carboxymethyl groups in the sugar backbone of CM-AbPS and conclude that the carboxymethyl groups mainly substitute on 4-position of the fructofuranose of the sugar backbone. The product has anti-tumor activity.

Key words: achyranthes bidentata, POLYSACCHARIDE, CARBOXYMETHYL GROUP, HPLC, ANTITUMOR DRUGS

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