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化学学报
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化学学报 ›› 2002, Vol. 60 ›› Issue (4): 674-680. 上一篇    下一篇

研究论文

配合物[Pd(L)(trp)]Cl·5H_2O(L=phen，5-NO_2phen)的合成、抗癌活性及其DNA作用研究

高恩君;刘祁涛   

  1. 沈阳化工学院配位化学研究室,沈阳(110021);辽宁大学化学科学与工程学院, 沈阳(110036)
  • 发布日期:2002-04-15

Study on the Synthesis, Anti-cancer Activity and the Interaction with DNA of Compounds [Pd(L)(trp)]Cl·5H_2O (L = phen, 5-NO_2phen)

Gao Enjun;Liu Qitao   

  1. Coordination Chemistry Section, Shenyang Institute of Chemical Technology,Shenyang(110021);Chemical Institute of Science and Technology, Liaoning University,Shenyang(110036)
  • Published:2002-04-15

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合成了两个混合配体配合物[Pd(phen)(trp)]Cl·5H_2O （1） 和 [Pd(5- NO_2phen)(trp)]Cl·5H_2O （2）（其中phen = 1，10-phenanthroline，5- NO_2phen = 5-nitro-1,10-phenanthroline，trp = L-tryptophan）。配合物对肺 腺癌细胞AGZY-83a均具有一定的杀伤活性，IC_(50)值分别为158.5 μg/mL和大于 293.0 μg/mL。荧光法测定配合物与鱼精DNA的结合常数（K）分别为6.36 * 10~6 和3.64 * 10~6。荧光光谱、紫外可见光谱和粘度法证实配合物主要以插入方式与 DNA结合。

关键词: 钯络合物, 抗癌药, DNA, 荧光分光光度法, 紫外分光光度法

[Pd(phen)(trp)]Cl·5H_2O and [Pd(5-NO_2phen)(trp)]Cl·5H_2O have been synthesized (phen = 1,10-phenanthroline, 5-NO_2phen = 5-nitro-1, 10-phenanthroline, trp = L-tryptophan). The compounds display anti- cancer activity to lung cancer cell AGZY-83a with IC_(50) of 158.5 μ g/mL and >293.0% μg/mL respectively. The binding constants of the compounds with sperm DNA are determined by fluorescence analysis, which are 6.36 * 10~6 and 3.64 * 10~6 respectively. Intercalation of the compounds with DNA can be demonstrated by fluorescence spectra, UV- spectra and viscometer.

Key words: PALLADIUM COMPLEX, ANTICARCINOGEN, DNA, FLUOROSPECTROPHOTOMETRY, UV

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