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化学学报
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化学学报 ›› 2003, Vol. 61 ›› Issue (6): 885-888. 上一篇    下一篇

研究论文

金属离子Cu~(2+)，Co~(2+)，Ni~(2+)的微芯片电泳分离及化学发光检测

苏荣国;林金明;屈锋;高云华;陈志锋   

  1. 中国科学院生态环境研究中心;中国科学院理化技术研究所
  • 发布日期:2003-06-15

Microchip Electropretic Separation of Cu~(2+), Co~(2+) and Ni~(2+) with Chemiluminescent Detection

Su Rongguo;Lin Jinming;Qu Feng;Gao Yunhua;Chen Zhifeng   

  1. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences;Technical Institute of Physics and Chemistry, Chinese Academy of Sciences
  • Published:2003-06-15

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根据流动注射－化学发光的通用实验装置图，设计并通过标准光刻技术，湿化 学刻蚀及热键合技术制作了玻璃微芯片。在该芯片上将毛细管电泳分离与化学发光 检测相联用，鲁米诺和H_2O_2化学发光反应溶液通过实验室自制的微流泵输送。对 所用电压，缓冲溶液和发光试剂流速等实验条件进行了优化。在所选的最佳条件下 ，成功地实现了金属离子Cu~(2+)，Co~(2+)，Ni~(2+)的电泳分离－化学发光检测， 过渡金属离子。

关键词: 流动注射分析, 化学发光分析, 鲁米诺, 过氧化氢, 铜离子, 钴离子, 镍离子

A glass microchip was designed on the base of flow injection- chemiluminescent devices and fabricated by standard photolithgraphy technology, wet chemical etching and heat bonding technology. Electrophoresis separation was cooperated with chemiluminescent detection on the microchip, and the chemiluminescent reagents of luminol and hydrogen peroxide solution were delivered by a laboratory- made microfluidic pump. The experimental conditions including buffer applied, voltage and the flux of the chemiluminescent reagents were optimized. Under the optimal conditions, Cu~(2+) , Co~(2+) and Ni~(2+) were separated and detected with chemiluminescent method on the chip. The detection limits of Cu~(2+) , Co~(2+) and Ni~(2+) can reach 5. O*10~(-9), 5.O*10~(-11) and 1.0*10~(-7) mol/L, respectively.

Key words: FLOW INJECTION ANALYSIS, CHEMILUMINESCENCE ANALYSIS, LUMINOL, HYDROGEN PEROXIDE, COPPER ION, COBALT ION, NICKEL ION

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