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化学学报
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化学学报 ›› 2004, Vol. 62 ›› Issue (12): 1153-1157. 上一篇    下一篇

研究论文

三维同步偏振荧光光谱研究蛋白质溶液变性时氨基酸残基的发光行为

唐波1, 杜鸣1, 陈蓁蓁1, 张慧1, 沈含熙2   

  1. 1. 山东师范大学化学化工与材料科学学院, 济南, 250014;
    2. 南开大学化学系, 天津, 300071
  • 投稿日期:2003-03-07 修回日期:2003-12-15 发布日期:2014-02-17
  • 通讯作者: 唐波,E-mail:tangb@sdnu.edu.cn;Fax:0531-6180017 E-mail:tangb@sdnu.edu.cn
  • 基金资助:
    国家自然科学基金(No.20335030)和山东省自然科学基金(No.Z2003B01)资助项目.

Studies on Luminescence of Trp and Tyr Residues in Protein Denaturation by Three-dimensional-synchronous-polarized Spectrofluorimetry

TANG Bo1, DU Ming1, CHEN Zhen-Zhen1, ZHANG Hui1, SHEN Han-Xi2   

  1. 1. College of Chemistry, Engineering and Materials Science, Shandong Normal University, Jinan 250014;
    2. Department of Chemistry, Nankai University, Tianjin 300071
  • Received:2003-03-07 Revised:2003-12-15 Published:2014-02-17

PDF

571

被引次数

61

将偏振技术、同步技术与三维技术结合起来的三维同步偏振荧光光谱(TDSPS)能分辨蛋白质溶液中的色氨酸(Trp)和酪氨酸(Tyr)残基,具有同步光谱分辨率高、三维技术信息丰富的优点.本文用TDSPS表征牛血清白蛋白(BSA)、人血清白蛋白(HSA)受各种因素影响(酸效应、碱效应、盐效应、猝灭剂等)时Trp,Tyr残基荧光光谱的变化,用于区分HSA和BSA.

关键词: 三维同步偏振荧光光谱, 色氨酸, 酪氨酸, 牛血清白蛋白, 人血清白蛋白

Based on polarizing, synchronous and three-dimensional technologies, a new three-dimensional-synchronous-polarized spectrofluorimetry (TDSPS) was used to distinguish tryptophan (Trp) and tyrosine (Tyr) residues in albumin solution with the advantages of abundant information of three-dimensional technology and high resolution of synchronous spectrofluorimetry. In this paper, the fluorescent spectral changes of Tyr and Trp residues in bovine serum albumin (BSA) and human serum albumin (HSA) were studied by obtaining the characteristic information that had been influenced by various factors (acidic, basic, salt and quenching reagents effects, etc.) with TDSPS. As a result, HSA and BSA could be distinguished.

Key words: three-dimensional-synchronous-polarized spectrofluorimetry(TDSPS), tryptophan(Trp), tyrosine(Tyr), bovine serum albumin(BSA), human serum albumin(HSA)