氨基安替比林分子原位烙印柱的制备及其水中结合性质的研究

化学学报 ›› 2004, Vol. 62 ›› Issue (12): 1176-1179. 上一篇下一篇

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氨基安替比林分子原位烙印柱的制备及其水中结合性质的研究

范子琳¹, 杨更亮^1,2, 张国庆¹, 刘海燕¹, 陈义²

1. 河北大学药学院, 保定, 071002;
2. 中国科学院化学研究所分子科学中心, 北京, 100080

投稿日期:2003-10-31 修回日期:2004-01-08 发布日期:2014-02-17
通讯作者: 杨更亮,E-mail:ygl@mail.hbu.edu.cn;Tel:0312-5079788 E-mail:ygl@mail.hbu.edu.cn
基金资助:
国家自然科学基金(No.20375010)、河北省自然科学基金(No.202096)、科学技术部基金(Nos.2002CB713803,KJCX-S5W-H06)、中国科学院"百人计划"和教育部优秀教师资助项目.

Aminoantipyrine Imprinted Column Prepared by in-situ Polymerization and Investigation of Binding Character in Aqueous Medium

FAN Zi-Lin¹, YANG Geng-Liang^1,2, ZHANG Guo-Qing¹, LIU Hai-Yan¹, CHEN Yi²

1. College of Pharmacy, Hebei University, Baoding 071002;
2. Molecular Science Center, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080

Received:2003-10-31 Revised:2004-01-08 Published:2014-02-17

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摘要/Abstract

实验采用原位聚合法以甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,偶氮二异丁腈(AIBN)为引发剂,正十二醇和甲苯为致孔剂直接在液相色谱柱中制备了氨基安替比林分子烙印柱.讨论了模板分子及其类似物在有机相和水相中的色谱行为及作用机理.实验表明,在水相中烙印柱具有很强的保留性能,氨基安替比林的保留因子可达到94.41.结果认为离子作用和疏水作用是使得氨基安替比林及类似物具有很强的保留性的重要原因,为研究模板聚合物的结合特性,采用前流分析法分别测定了氨基安替比林、氨基比林和安替比林在烙印柱上的吸附等温线.实验表明,分子烙印聚合物对烙印分子表现了较高的选择性,这和通过比较各物质在分子烙印柱和空白柱上保留因子所获得的结果一致.

关键词: 分子烙印, 水相识别, 原位聚合, 氨基安替比林

Aminoantipyrine imprinted column was prepared by in situ polymerization in a 50 mm×4.6 mm I D. stainless steel column. Methacrylic acid (MAA), ethylene glycol dimethacrylate (EDMA), 2,2'-azobisisobutyronitrile (AIBN) were used as the monomer, crosslinker and initiator, respectively. Dodecanol and toluene were used as porogen. Both organic and aqueous mobile phases were applied for the evaluation of the chromatographic character of the column, and the mechanism of molecular recognition was discussed. It was found that the imprinted column showed high selectivity towards aminoantipyrine in aqueous medium. Ionic and hydrophobic interactions were presumed as the reasons for the molecule recognition in aqueous medium. Under the optimum condition, the retention factor of aminoantipyrine could achieve 94 41. Rectangular pulse frontal analysis method was adopted to determine the single component absorption isotherms. In contrast with the retention factors gained from molecular imprinting polymers, the accordant results were obtained.

Key words: molecular imprinting, recognition in aqueous medium, in-situ polymerization, aminoantipyrine

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范子琳, 杨更亮, 张国庆, 刘海燕, 陈义. 氨基安替比林分子原位烙印柱的制备及其水中结合性质的研究[J]. 化学学报, 2004, 62(12): 1176-1179.

FAN Zi-Lin, YANG Geng-Liang, ZHANG Guo-Qing, LIU Hai-Yan, CHEN Yi. Aminoantipyrine Imprinted Column Prepared by in-situ Polymerization and Investigation of Binding Character in Aqueous Medium[J]. Acta Chimica Sinica, 2004, 62(12): 1176-1179.

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参考文献

[1] Shea, K. J.; Stoddard, G. J.; Shavelle, D. M.; Wakul, F.; Choate, R. M. Macromolecules 1990, 23, 4497.
[2] Wullf, G.; Sarhan, A. Angew. Chem. 1972, 11, 341.
[3] Yamazaki, T.; Yilmaz, E.; Mosbach, K.; Sode, K. Anal. Chim. Acta 2001, 435, 209.
[4] Theodoridis, G.; Manesiotis, P. J. Chromatogr., A 2002, 948, 163.
[5] Vlatakis, G.; Andersson, L. I.; Muller, R.; Mosbach, K. Nature 1993, 361, 645.
[6] Bereozki, A.; Tolokan, A.; Horvai, G.; Horvath, V.; Lanza, F.; Hall, A. J.; Sellergern, B. J. Chromatogr., A 2001, 930, 31.
[7] Hosoya, K.; Yoshizako, K.; Shirasu, Y.; Kimata, K.; Araki, T.; Tanaka, N.; Haginaka, J. J. Chromatogr., A 1996, 728, 139.
[8] Toorisake, E.; Uezu, K.; Goto, M.; Furusaki, S. Biochem. Eng. J. 2003, 14, 85.
[9] Yoshikawa, M.; Izumi, J.; Kitao, T. Chem. Lett. 1996, 8, 611.
[10] Dickert, F. L.; Forth, P.; Lieberzeit, P.; Tortschanoff, M. Fresenius J. Anal. Chem. 1998, 360, 759.
[11] Andersson, L. I. Anal. Chem. 1996, 68, 111.
[12] Fan, Z.-L.; Yang, G.-L.; Liu, H.-Y.; Chen, Y. Chin. J. Chromatogr. 2003, 21, 199 (in Chinese).(范子琳, 杨更亮, 刘海燕, 陈义, 色谱, 2003, 21, 199.)
[13] Matsui, J.; Nicholls, I. A.; Takeuchi, T. Anal. Chim. Acta 1998, 365, 89.
[14] Zhou, J.; Wang, S.-W.; Guo, H.-S.; He, X.-W. Chin. J. Analy. Chem. 2000, 28, 296 (in Chinese).(周杰, 王善伟, 郭洪声, 何锡文, 分析化学, 2000, 28, 296.)
[15] Huang, X.-D.; Zou, H.-F.; Chen, X.-M.; Luo, Q.-Z.; Kong, L. J. Chromatogr., A 2003, 984, 273.
[16] Rosatzin, T.; Andersson, L. I.; Simon, W.; Mosbach, K. J. Chem. Soc., Perkin Trans. 2 1991, 1261.
[17] Kugimiya, A.; Matsui, J.; Takeuchi, T.; Yano, K.; Muguruma, H.; Elgersma, A. V.; Karube, I. Anal. Lett. 1996, 29, 1099.
[18] Guo, H. S.; He, X. W. Fresenius′ J. Anal. Chem. 2000, 368, 461.
[19] Yang, G. L. Chromatographia 1999, 50, 621.
[20] Martin-Esteban, A. Fresenius J. Anal. Chem. 2001, 370, 795.
[21] Ansell, R. J.; Mosbach, K. J. Chromatogr., A 1997, 787, 55.
[22] Matsui, J.; Kubo, H.; Takeuchi, T. Anal. Sci. 1998, 14, 699.
[23] Berglund, J.; Lindbladh, C.; Nicholls, I. A.; Mosbach, K. Anal. Commun. 1998, 35, 3.
[24] Haupt, K.; Dzgoev, A.;Mosbach, K. Anal. Chem. 1998, 70, 628.

氨基安替比林分子原位烙印柱的制备及其水中结合性质的研究

Aminoantipyrine Imprinted Column Prepared by in-situ Polymerization and Investigation of Binding Character in Aqueous Medium

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